一项关于童年期受过创伤的老年人复杂创伤后症状相关DNA甲基化修饰的初步调查。
A pilot investigation on DNA methylation modifications associated with complex posttraumatic symptoms in elderly traumatized in childhood.
作者信息
Marinova Zoya, Maercker Andreas, Grünblatt Edna, Wojdacz Tomasz K, Walitza Susanne
机构信息
Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric Hospital, University of Zurich, Zurich, Switzerland.
Department of Psychology, Division of Psychopathology and Clinical Intervention, University of Zurich, Binzmühlerstrasse 14/17, Raum BIN 3 E 14, 8050, Zurich, Switzerland.
出版信息
BMC Res Notes. 2017 Dec 19;10(1):752. doi: 10.1186/s13104-017-3082-y.
OBJECTIVE
Complex posttraumatic stress disorder (CPTSD) is a newly proposed diagnosis in the International Classification of Diseases-version 11, which is currently intensively investigated. Childhood trauma is regarded as main source of CPTSD symptoms, even in later life. Induction of DNA methylation changes by childhood trauma may contribute to its long-lasting adverse health consequences. The current study analyzed the correlation of genome-wide DNA methylation profiles with complex posttraumatic sequelae in buccal epithelial cells from 31 elderly former indentured child laborers (Verdingkinder) using the Infinium Illumina 450k Human DNA methylation chip.
RESULTS
DNA methylation modifications indicated experiment-wide significant associations with the following complex posttraumatic symptom domains: dissociation, tension reduction behavior and dysfunctional sexual behavior. Differentially methylated CpG sites were mapped to the genes huntington associated protein 1 (HAP1), RAN binding protein 2 (RANBP2) and proteasome subunit alpha 4 (PSMA4), respectively. In addition, the methylation of cg07225277 located in carnosine synthase 1 (CARNS1) correlated with trauma symptom complexity. Our pilot data suggest correlation of DNA methylation modifications with complex posttraumatic symptoms in elderly individuals subjected to prolonged and complex childhood trauma. More comprehensive and elaborated studies should be carried out to analyze epigenetic modifications associated with CPTSD.
目的
复杂性创伤后应激障碍（CPTSD）是《国际疾病分类第11版》中新提出的一种诊断，目前正在深入研究。童年创伤被视为CPTSD症状的主要来源，即使在晚年也是如此。童年创伤引起的DNA甲基化变化可能导致其长期不良健康后果。本研究使用Illumina 450k人类DNA甲基化芯片，分析了31名老年前契约童工（Verdingkinder）颊上皮细胞中全基因组DNA甲基化谱与复杂性创伤后遗症之间的相关性。
结果
DNA甲基化修饰表明在全实验范围内与以下复杂性创伤后症状领域存在显著关联：解离、紧张缓解行为和性功能障碍行为。差异甲基化的CpG位点分别映射到亨廷顿相关蛋白1（HAP1）、RAN结合蛋白2（RANBP2）和蛋白酶体亚基α4（PSMA4）基因。此外，肌肽合酶1（CARNS1）中cg07225277的甲基化与创伤症状复杂性相关。我们的初步数据表明，在经历长期和复杂童年创伤的老年人中，DNA甲基化修饰与复杂性创伤后症状相关。应开展更全面、详细的研究来分析与CPTSD相关的表观遗传修饰。
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