Parsons Paul J, Couchoux Christelle, Horsburgh Gavin J, Dawson Deborah A, Field Jeremy
Centre for Ecology and Conservation, University of Exeter, Penryn Campus, Cornwall, TR10 9EZ, UK.
NERC Biomolecular Analysis Facility, Department of Animal and Plant Sciences, University of Sheffield, Western Bank, Sheffield, S10 2TN, UK.
BMC Res Notes. 2017 Dec 19;10(1):753. doi: 10.1186/s13104-017-3089-4.
The objective here is to identify highly polymorphic microsatellite loci for the Palaearctic sweat bee Lasioglossum malachurum. Sweat bees (Hymenoptera: Halictidae) are widespread pollinators that exhibit an unusually large range of social behaviours from non-social, where each female nests alone, to eusocial, where a single queen reproduces while the other members of the colony help to rear her offspring. They thus represent excellent models for understanding social evolution.
24 new microsatellite loci were successfully optimized. When amplified across 23-40 unrelated females, the number of alleles per locus ranged from 3 to 17 and the observed heterozygosities 0.45 to 0.95. Only one locus showed evidence of significant deviation from Hardy-Weinberg equilibrium. No evidence of linkage disequilibrium was found. These 24 loci will enable researchers to gain greater understanding of colony relationships within this species, an important model for the study of eusociality. Furthermore, 22 of the same loci were also successfully amplified in L. calceatum, suggesting that these loci may be useful for investigating the ecology and evolution of sweat bees in general.
本研究旨在为古北区汗蜂黄斑切叶蜂鉴定高度多态的微卫星位点。汗蜂（膜翅目：隧蜂科）是广泛分布的传粉者，表现出从非社会性（每个雌蜂单独筑巢）到真社会性（单个蜂后繁殖，而蜂群中的其他成员帮助抚养其后代）等异常广泛的社会行为范围。因此，它们是理解社会进化的优秀模型。
成功优化了24个新的微卫星位点。当在23 - 40只无亲缘关系的雌蜂中进行扩增时，每个位点的等位基因数从3到17不等，观察到的杂合度为0.45至0.95。只有一个位点显示出显著偏离哈迪 - 温伯格平衡的证据。未发现连锁不平衡的证据。这24个位点将使研究人员能够更深入地了解该物种内的蜂群关系，这是研究真社会性的一个重要模型。此外，相同的22个位点在黄胫切叶蜂中也成功扩增，这表明这些位点可能总体上有助于研究汗蜂的生态学和进化。
