Shahin Rima, Iwanaga Masashi, Kawasaki Hideki
Faculty of Agriculture, Utsunomiya University, 350 Mine, Utsunomiya, Tochigi 321-8505, Japan.
Faculty of Agriculture, Utsunomiya University, 350 Mine, Utsunomiya, Tochigi 321-8505, Japan.
Gene. 2018 Mar 10;646:181-194. doi: 10.1016/j.gene.2017.11.076. Epub 2017 Dec 16.
We aimed to clarify the regulation of cuticular protein (CP) gene expression and the resulting insect cuticular layers by comparing the expression pattern of CP genes and related ecdysone-responsive transcription factor (ERTF) genes, the coding amino acid sequences of CP genes, and histological observation. The expression of CP and ERTF genes during pupal and adult stages was examined via qPCR. The number of CP genes expressed during pupal and adult stages decreased as compared to that during prepupal to pupation stages, particularly in CPRs. The peaks of transcripts were observed at P5, P6, P9, A0, and A1. The order of the ERTF and CP genes expression resembled that at prepupal and pupation stages, suggesting the relatedness of ERTFs with the same CP genes at both stages. Moreover, the order of expression of CP genes resembled that in prepupal to pupation stages, by which we presumed the spaces of CPs in the epicuticle, outer-exocuticle, inner-exocuticle, endocuticle layer.
我们旨在通过比较表皮蛋白（CP）基因和相关蜕皮激素应答转录因子（ERTF）基因的表达模式、CP基因的编码氨基酸序列以及组织学观察，来阐明表皮蛋白（CP）基因表达的调控以及由此产生的昆虫表皮层。通过qPCR检测了CP和ERTF基因在蛹期和成虫期的表达情况。与预蛹期到化蛹期相比，蛹期和成虫期表达的CP基因数量减少，尤其是在CPRs中。在P5、P6、P9、A0和A1观察到转录本峰值。ERTF和CP基因的表达顺序与预蛹期和化蛹期相似，表明两个阶段中ERTF与相同CP基因的相关性。此外，CP基因的表达顺序与预蛹期到化蛹期相似，据此我们推测了CPs在表皮、外皮层外层、外皮层内层、内皮层中的空间分布。
