Department of Breast Cancer Pathology and Research Laboratory, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.
Department of Tumor Cell Biology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, China.
FASEB J. 2018 May;32(5):2395-2410. doi: 10.1096/fj.201700825RR. Epub 2017 Dec 19.
Girdin is an actin-binding protein playing key roles in the development of various carcinomas. Although online tools have predicted nuclear localization of girdin with a high probability, convincing proof has rarely been provided until now. The purpose of this study was to discover girdin's precise subcellular distribution and the potential prognostic value corresponding to its localization. The subcellular distribution of girdin was detected in a human breast cancer cell line and in >800 samples of human breast tissue by clinical pathologic analysis. In this study, we discovered for the first time that girdin could attach to chromatin and interact with topoisomerase-IIα in nucleus. Cytoplasmic and nuclear girdin exhibited different roles in prognosis of breast cancer: cytoplasmic girdin expression was an independent prognostic factor for progression-free survival (PFS), whereas nuclear girdin expression was an independent prognostic factor for overall survival (OS). More important, combination cytoplasmic and nuclear girdin was an independent prognosis factor of both OS and PFS. In conclusion, our research results strongly recommend combination analysis of cytoplasmic and nuclear girdin for a precise prognostic prediction in breast cancer.-Zhang, H., Yu, F., Qin, F., Shao, Y., Chong, W., Guo, Z., Liu, X., Fu, L., Gu, F., Ma, Y. Combination of cytoplasmic and nuclear girdin expression is an independent prognosis factor of breast cancer.
Girdin 是一种肌动蛋白结合蛋白，在多种癌症的发生发展中发挥关键作用。尽管在线工具预测 Girdin 具有很高的核定位概率，但到目前为止，很少有令人信服的证据来证明这一点。本研究旨在发现 Girdin 的精确亚细胞分布及其定位对应的潜在预后价值。通过临床病理分析，在人乳腺癌细胞系和 >800 个人类乳腺癌组织样本中检测 Girdin 的亚细胞分布。在这项研究中，我们首次发现 Girdin 可以附着在染色质上，并与核内拓扑异构酶-IIα相互作用。细胞质和核 Girdin 在乳腺癌的预后中表现出不同的作用：细胞质 Girdin 表达是无进展生存期（PFS）的独立预后因素，而核 Girdin 表达是总生存期（OS）的独立预后因素。更重要的是，细胞质和核 Girdin 的联合表达是 OS 和 PFS 的独立预后因素。总之，我们的研究结果强烈建议联合分析细胞质和核 Girdin，以对乳腺癌进行精确的预后预测。-Zhang, H., Yu, F., Qin, F., Shao, Y., Chong, W., Guo, Z., Liu, X., Fu, L., Gu, F., Ma, Y. 细胞质和核 Girdin 表达的联合是乳腺癌的独立预后因素。
