Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA 50011;
Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093.
Proc Natl Acad Sci U S A. 2018 Jan 2;115(1):210-215. doi: 10.1073/pnas.1717519115. Epub 2017 Dec 19.
Lysine acetylation is a key posttranslational modification that regulates diverse proteins involved in a range of biological processes. The role of histone acetylation in plant defense is well established, and it is known that pathogen effector proteins encoding acetyltransferases can directly acetylate host proteins to alter immunity. However, it is unclear whether endogenous plant enzymes can modulate protein acetylation during an immune response. Here, we investigate how the effector molecule HC-toxin (HCT), a histone deacetylase inhibitor produced by the fungal pathogen race 1, promotes virulence in maize through altering protein acetylation. Using mass spectrometry, we globally quantified the abundance of 3,636 proteins and the levels of acetylation at 2,791 sites in maize plants treated with HCT as well as HCT-deficient or HCT-producing strains of Analyses of these data demonstrate that acetylation is a widespread posttranslational modification impacting proteins encoded by many intensively studied maize genes. Furthermore, the application of exogenous HCT enabled us to show that the activity of plant-encoded enzymes (histone deacetylases) can be modulated to alter acetylation of nonhistone proteins during an immune response. Collectively, these results provide a resource for further mechanistic studies examining the regulation of protein function by reversible acetylation and offer insight into the complex immune response triggered by virulent .
赖氨酸乙酰化是一种关键的翻译后修饰，可调节参与多种生物过程的各种蛋白质。组蛋白乙酰化在植物防御中的作用已得到充分证实，已知病原体效应蛋白编码乙酰转移酶可以直接乙酰化宿主蛋白以改变免疫。然而，尚不清楚内源性植物酶是否可以在免疫反应期间调节蛋白质乙酰化。在这里，我们研究了真菌病原体 race 1 产生的组蛋白去乙酰化酶抑制剂 HC 毒素 (HCT) 如何通过改变蛋白质乙酰化来促进玉米的毒力。使用质谱法，我们全局定量了玉米植物中 HCT 处理以及 HCT 缺陷或 HCT 产生的 菌株中 3636 种蛋白质的丰度和 2791 个位点的乙酰化水平。对这些数据的分析表明，乙酰化是一种广泛存在的翻译后修饰，影响许多经过深入研究的玉米基因编码的蛋白质。此外，外源性 HCT 的应用使我们能够表明，植物编码酶（组蛋白去乙酰化酶）的活性可以被调节，以在免疫反应期间改变非组蛋白蛋白质的乙酰化。总的来说，这些结果为进一步研究通过可逆乙酰化调节蛋白质功能的机制提供了资源，并深入了解了由毒力引发的复杂免疫反应。
