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鉴定未成熟和成熟大鼠前垂体中的长非编码 RNA。

Identification of long non-coding RNAs in the immature and mature rat anterior pituitary.

机构信息

Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, 130062, Jilin, P.R. China.

出版信息

Sci Rep. 2017 Dec 19;7(1):17780. doi: 10.1038/s41598-017-17996-6.

DOI:10.1038/s41598-017-17996-6
PMID:29259254
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5736705/
Abstract

Many long non-coding RNAs (lncRNAs) have been identified in several types of human pituitary adenomas and normal anterior pituitary, some of which are involved in the pathogenesis of pituitary adenomas. However, a systematic analysis of lncRNAs expressed at different developmental stages of normal pituitary, particularly in rats, has not been performed. Therefore, we contrasted two cDNA libraries of immature (D15) and mature (D120) anterior pituitary in rat that were sequenced on an Illumina HiSeq Xten platform, and a total of 29,568,806,352 clean reads were identified. Notably, 7039 lncRNA transcripts corresponded to 4442 lncRNA genes, and 1181 lncRNA transcripts were significantly differentially expressed in D15 and D120. In addition, 6839 protein-coding genes (<100 kb upstream and downstream) were the nearest neighbors of 4074 lncRNA genes. An interaction network of lncRNAs and the follicle-stimulating hormone beta-subunit (FSHb) gene was constructed using the lncRNATargets platform, and three novel lncRNAs were obtained. Furthermore, we detected the expression of the novel lncRNAs and ten highly expressed lncRNAs that were randomly selected through quantitative PCR (qPCR). The rat anterior pituitary lncRNA content identified in this study provides a more in-depth understanding of the roles of these lncRNAs in hormone and reproduction development and regulation in mammals.

摘要

许多长链非编码 RNA（lncRNA）已在几种类型的人类垂体腺瘤和正常垂体前叶中被鉴定出来，其中一些与垂体腺瘤的发病机制有关。然而，尚未对正常垂体不同发育阶段表达的 lncRNA 进行系统分析，特别是在大鼠中。因此，我们对比了两个处于不成熟（D15）和成熟（D120）期的大鼠垂体前叶 cDNA 文库，文库测序在 Illumina HiSeq Xten 平台上进行，共鉴定出 29,568,806,352 条清洁读段。值得注意的是，7039 个 lncRNA 转录本对应于 4442 个 lncRNA 基因，1181 个 lncRNA 转录本在 D15 和 D120 之间差异显著表达。此外，6839 个（上下游距离小于 100kb）蛋白编码基因是 4074 个 lncRNA 基因的最近邻。使用 lncRNATargets 平台构建了 lncRNA 和卵泡刺激素β亚基（FSHb）基因的相互作用网络，并获得了三个新的 lncRNA。此外，我们通过 qPCR 检测了新的 lncRNA 和随机选择的 10 个高表达 lncRNA 的表达。本研究中鉴定的大鼠垂体前叶 lncRNA 含量为深入了解这些 lncRNA 在激素和生殖发育以及哺乳动物调节中的作用提供了更多的信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4242/5736705/a5144129bc5a/41598_2017_17996_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4242/5736705/941536c6451d/41598_2017_17996_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4242/5736705/10def98004b7/41598_2017_17996_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4242/5736705/2f99012a7d30/41598_2017_17996_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4242/5736705/846000847f46/41598_2017_17996_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4242/5736705/bfce7a81c881/41598_2017_17996_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4242/5736705/a5144129bc5a/41598_2017_17996_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4242/5736705/941536c6451d/41598_2017_17996_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4242/5736705/10def98004b7/41598_2017_17996_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4242/5736705/2f99012a7d30/41598_2017_17996_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4242/5736705/846000847f46/41598_2017_17996_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4242/5736705/bfce7a81c881/41598_2017_17996_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4242/5736705/a5144129bc5a/41598_2017_17996_Fig6_HTML.jpg

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