Sakhaie Mohammad Hassan, Soleimani Mansoureh, Pourheydar Bagher, Majd Zahra, Atefimanesh Pezhman, Asl Sara Soleimani, Mehdizadeh Mehdi
Cellular and Molecular Research Center and Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran.
Department of Anatomy, Arak University of Medical Sciences, Arak, Iran.
Behav Neurol. 2017;2017:9194261. doi: 10.1155/2017/9194261. Epub 2017 Nov 12.
Exposure to extremely low-frequency electromagnetic fields may induce constant modulation in neuronal plasticity. In recent years, tremendous efforts have been made to design a suitable strategy for enhancing adult neurogenesis, which seems to be deterred due to brain senescence and several neurodegenerative diseases. In this study, we evaluated the effects of ELF-EMF on neurogenesis and memory, following treatment with trimethyltin chloride (TMT) as a neurotoxicant. The mice in all groups ( = 56) were injected with BrdU during the experiment for seven consecutive days to label newborn cells. Spatial memory was assessed by the Morris water maze (MWM) test. By the end of the experiment, neurogenesis and neuronal differentiation were assessed in the hippocampus, using immunohistochemistry and Western blot analysis. Based on the findings, exposure to ELF-EMF enhanced spatial learning and memory in the MWM test. ELF-EMF exposure significantly enhanced the number of BrdU+ and NeuN+ cells in the dentate gyrus of adult mice ( < 0.001 and < 0.05, resp.). Western blot analysis revealed significant upregulation of NeuroD2 in ELF-EMF-exposed mice compared to the TMT-treated group ( < 0.05). These findings suggest that ELF-EMF might have clinical implications for the improvement of neurodegenerative processes and could help develop a novel therapeutic approach in regenerative medicine.
暴露于极低频电磁场可能会引起神经元可塑性的持续调节。近年来，人们付出了巨大努力来设计一种合适的策略以增强成体神经发生，然而由于脑衰老和几种神经退行性疾病，这一过程似乎受到了阻碍。在本研究中，我们评估了极低频电磁场对神经发生和记忆的影响，实验中使用氯化三甲基锡（TMT）作为神经毒素进行处理。所有组（ = 56）的小鼠在实验期间连续七天注射溴脱氧尿苷（BrdU）以标记新生细胞。通过莫里斯水迷宫（MWM）测试评估空间记忆。在实验结束时，使用免疫组织化学和蛋白质印迹分析评估海马体中的神经发生和神经元分化。基于这些发现，在MWM测试中，暴露于极低频电磁场可增强空间学习和记忆。暴露于极低频电磁场显著增加了成年小鼠齿状回中BrdU+和NeuN+细胞的数量（分别为 < 0.001和 < 0.05）。蛋白质印迹分析显示，与TMT处理组相比，暴露于极低频电磁场的小鼠中NeuroD2显著上调（ < 0.05）。这些发现表明，极低频电磁场可能对改善神经退行性过程具有临床意义，并有助于开发再生医学中的新型治疗方法。
