一个更大的巧克力芯片——开发一个15K的L. SNP阵列以创建高密度连锁图谱。

A Larger Chocolate Chip-Development of a 15K L. SNP Array to Create High-Density Linkage Maps.

作者信息

Livingstone Donald, Stack Conrad, Mustiga Guiliana M, Rodezno Dayana C, Suarez Carmen, Amores Freddy, Feltus Frank A, Mockaitis Keithanne, Cornejo Omar E, Motamayor Juan C

机构信息

Mars, Inc., Miami, FL, United States.

Estación Experimental Tropical Pichilingue, National Institute of Agricultural Research, Quevedo, Ecuador.

出版信息

Front Plant Sci. 2017 Dec 5;8:2008. doi: 10.3389/fpls.2017.02008. eCollection 2017.

Cacao ( L.) is an important cash crop in tropical regions around the world and has a rich agronomic history in South America. As a key component in the cosmetic and confectionary industries, millions of people worldwide use products made from cacao, ranging from shampoo to chocolate. An Illumina Infinity II array was created using 13,530 SNPs identified within a small diversity panel of cacao. Of these SNPs, 12,643 derive from variation within annotated cacao genes. The genotypes of 3,072 trees were obtained, including two mapping populations from Ecuador. High-density linkage maps for these two populations were generated and compared to the cacao genome assembly. Phenotypic data from these populations were combined with the linkage maps to identify the QTLs for yield and disease resistance.

可可（Theobroma cacao L.）是世界热带地区一种重要的经济作物，在南美洲有着丰富的农艺学历史。作为化妆品和糖果行业的关键成分，全球数百万人使用由可可制成的产品，从洗发水到巧克力。利用在一个小型可可多样性群体中鉴定出的13530个单核苷酸多态性（SNP）创建了一个Illumina Infinity II阵列。在这些SNP中，12643个来自注释可可基因内的变异。获得了3072棵树的基因型，包括来自厄瓜多尔的两个作图群体。为这两个群体生成了高密度连锁图谱，并与可可基因组组装进行比较。将这些群体的表型数据与连锁图谱相结合，以鉴定产量和抗病性的数量性状位点（QTL）。