Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, 1, Sec. 3, Zhongxiao E. Rd., Taipei 10608, Taiwan.
Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, No. 35, Keyan Road, Zhunan Town, Miaoli County 35053, Taiwan.
Biomed Res Int. 2017;2017:7124817. doi: 10.1155/2017/7124817. Epub 2017 Nov 13.
Many external stimulations have been shown to promote bone regeneration. The effects of an alternating current (AC) electrostatic field, one of external stimulations, generated from a device with high voltage and low current output on human osteoblastic cell line have been investigated in this study. We investigated how human osteoblasts responded to an AC electrostatic field, and the output parameters were set as 1 kV and 160 A. Our results showed that, under such condition, the AC electrostatic field had a downregulation effect on the production ability of alkaline phosphatase and type 1 collagen expression. However, the expression of osteocalcin gene was elevated on the end of EFID treatment suggesting that AC electrostatic field might be a potential stimulation for accelerating the differentiation of osteoblastic cells.
许多外部刺激已被证明可促进骨再生。本研究旨在探讨一种由高压低电流输出装置产生的交流（AC）静电场作为外部刺激之一，对人成骨细胞系的影响。我们研究了人成骨细胞对 AC 静电场的反应，输出参数设定为 1 kV 和 160 A。结果表明，在此条件下，AC 静电场对碱性磷酸酶的产生能力和 I 型胶原表达的下调作用。然而，EFID 治疗结束时骨钙素基因的表达升高，提示 AC 静电场可能是促进成骨细胞分化的潜在刺激。
