Van Hoeyveld E M, Lejoly M, Walravens M J, Stevens E A
Department of Medicine, University Hospitals, University of Leuven, Belgium.
J Allergy Clin Immunol. 1989 Mar;83(3):601-9. doi: 10.1016/0091-6749(89)90072-9.
The effect of epsilon-aminocaproic acid (EACA) on the degradation of aqueous pollen extracts was studied by isoelectric focusing, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunoblotting. The extracts were stored at 4 degrees C for 7 days and at 37 degrees C for 1, 4, and 7 days. Addition of 0.1 mol/L of EACA before storage partly protected the extract from degradation. The protective effect of EACA could be demonstrated most clearly by immunoblotting, suggesting that more epitopes were preserved in an antigenic configuration. The stabilizing effect increased with higher EACA concentrations.
通过等电聚焦、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和免疫印迹法研究了ε-氨基己酸（EACA）对水性花粉提取物降解的影响。提取物分别在4℃下储存7天，以及在37℃下储存1天、4天和7天。储存前添加0.1mol/L的EACA可部分保护提取物不被降解。EACA的保护作用通过免疫印迹法最能清楚地证明，这表明更多的表位以抗原形式得以保留。随着EACA浓度的升高，稳定作用增强。
