Oskay Yiğit, Çetin Barbaros, Şerifoğlu Naz, Arslan-Ergül Ayça, Adams Michelle M
1 Mechanical Engineering Department, İ.D. Bilkent University , Ankara, Turkey .
2 Interdisciplinary Graduate Program in Neuroscience, Aysel Sabuncu Brain Research Center, Bilkent University , Ankara, Turkey .
Zebrafish. 2018 Apr;15(2):85-95. doi: 10.1089/zeb.2017.1513. Epub 2017 Dec 20.
In this study, we designed and developed a novel low-cost system for anesthetizing and injecting adult zebrafish. The system utilizes a gradual cooling method for the anesthesia and maintains the fish in a stable anesthetic plane, as well as stabilizes the animal so that intraperitoneal injections can be consistently performed. It is a system that any laboratory with access to a workshop can build for their group. Moreover, it is a safe system for researchers, as well as a reliable one for repeated experiments since multiple fish can be injected quickly and there is little physical contact necessary between the investigator and the animal. This will likely reduce any unnecessary stress in the fish, as compared with manual methods of injection. Finally, the system is adaptable so that as the investigators' procedural needs change due to different research questions, that is, gradual rewarming or something of that nature, it could be modified.
在本研究中，我们设计并开发了一种用于麻醉和注射成年斑马鱼的新型低成本系统。该系统采用逐步冷却法进行麻醉，并使鱼维持在稳定的麻醉平面，同时稳定动物，以便能够持续进行腹腔注射。这是一个任何能够进入车间的实验室都可以为其团队构建的系统。此外，它对研究人员来说是一个安全的系统，并且由于可以快速注射多条鱼，研究人员与动物之间几乎无需身体接触，所以对于重复实验来说也是一个可靠的系统。与手动注射方法相比，这可能会减少鱼的任何不必要应激。最后，该系统具有适应性，以便随着研究人员因不同研究问题而导致的程序需求变化，即逐步复温或类似性质的情况，它可以进行修改。
