Ciocan Răzvan A, Drugan Cristina, Gherman Claudia D, Cătană Cristina-Sorina, Ciocan Andra, Drugan Tudor C, Bolboacă Sorana D
Department of Medical Informatics and Biostatistics, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Department of Vascular Surgery, Second Surgical Clinic, Emergency County Hospital "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Ann Clin Lab Sci. 2017 Nov;47(6):713-719.
Chitotriosidase is an enzyme secreted by activated macrophages. This study aims to investigate the usefulness of circulating chitotriosidase activity as a marker of inflammatory status in patients with critical limb ischemia (CLI).
An observational gender-matched case-control study was conducted on patients hospitalized with the primary diagnosis of CLI, as well as a control group. The control group consisted of healthy volunteers.
Forty-three patients were included in each group. Similar demographic characteristics (median age of 60-62 years and overweight) were observed in both groups. Chitotriosidase activity ranged from 110 nmol/ml/hr to 1530 nmol/ml/hr in the CLI group and from 30 nmol/ml/hr to 440 nmol/ml/hr in the control group; demonstrating significantly elevated values in the CLI group (<0.001). Median plasma chitotriosidase activity was significantly elevated in smokers compared with non-smokers in both groups (<0.05). However, this activity had higher values in CLI than in control subjects. Receiver operating characteristic (ROC) analysis was then performed in order to verify the diagnostic accuracy of chitotriosidase as an inflammatory biomarker in CLI.
Circulating chitotriosidase is a test which can potentially be used for the monitoring of CLI patients without other inflammatory conditions. However, the interpretation of elevated values must take into account the inflammatory response induced by tobacco exposure.
壳三糖苷酶是一种由活化巨噬细胞分泌的酶。本研究旨在探讨循环壳三糖苷酶活性作为严重肢体缺血（CLI）患者炎症状态标志物的实用性。
对以CLI为主要诊断入院的患者以及一个对照组进行了一项性别匹配的观察性病例对照研究。对照组由健康志愿者组成。
每组纳入43例患者。两组观察到相似的人口统计学特征（中位年龄60 - 62岁且超重）。CLI组壳三糖苷酶活性范围为110 nmol/ml/hr至1530 nmol/ml/hr，对照组为30 nmol/ml/hr至440 nmol/ml/hr；CLI组的值显著升高（<0.001）。两组中，吸烟者的血浆壳三糖苷酶活性中位数与非吸烟者相比均显著升高（<0.05）。然而，CLI组的该活性值高于对照组。随后进行了受试者工作特征（ROC）分析，以验证壳三糖苷酶作为CLI炎症生物标志物的诊断准确性。
循环壳三糖苷酶是一种可潜在用于监测无其他炎症情况的CLI患者的检测方法。然而，对升高值的解读必须考虑烟草暴露引起的炎症反应。
