Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Laboratory affiliated with Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Rome, Italy.
Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Laboratory affiliated with Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Rome, Italy
Antimicrob Agents Chemother. 2018 Feb 23;62(3). doi: 10.1128/AAC.01820-17. Print 2018 Mar.
Lipid A aminoarabinosylation is invariably associated with colistin resistance in ; however, the existence of alternative aminoarabinosylation-independent colistin resistance mechanisms in this bacterium has remained elusive. By combining reverse genetics with experimental evolution assays, we demonstrate that a functional lipid A aminoarabinosylation pathway is critical for the acquisition of colistin resistance in reference and clinical isolates. This highlights lipid A aminoarabinosylation as a promising target for the design of colistin adjuvants against .
脂质 A 氨基阿拉伯糖基化与 ; 中的多粘菌素耐药性密切相关；然而，这种细菌中是否存在其他氨基阿拉伯糖基化非依赖性的多粘菌素耐药机制仍不清楚。通过将反向遗传学与实验进化测定相结合，我们证明了功能性脂质 A 氨基阿拉伯糖基化途径对于参考株和临床分离株获得多粘菌素耐药性至关重要。这凸显了脂质 A 氨基阿拉伯糖基化作为设计多粘菌素佐剂的有前途的靶标。
