Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, P.R. China.
Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Hangzhou, 310058, P.R. China.
Sci Rep. 2017 Dec 20;7(1):17930. doi: 10.1038/s41598-017-17743-x.
To better understand the relationship between the environmental variables and microbial communities of activated sludge, we took winter samples from different biological treatment units (anaerobic, oxic, etc) from the WWTP's of a number of Chinese cities. Differences in influent organic components and activated sludge microbial communities were identified by gas chromatography-mass spectrometry and high-throughput sequencing technology, respectively. Liquid nitrogen grinding pretreatment of samples was found to aid in the obtaining of a more bio-diversified sample. Influent type and dissolved oxygen concentration influenced the activated sludge microbial community structure. Nitrospira, Caldilineaceae and Anaerolineaceae were highly related to domestic wastewater treatment systems, whereas Thauera was the most abundant putative refractory aromatic hydrocarbon decomposer found in industrial wastewater treatment systems. Within the influent composition, we speculate that Thauera, Macellibacteroides and Desulfomicrobium are the key functional genera of the anaerobic environment of the textile dyeing industry wastewater treatment systems, whilst Thauera and Thiobacillus are key functional microbes in fine chemical wastewater treatment systems.
为了更好地理解环境变量与活性污泥微生物群落之间的关系，我们从中国多个城市的污水处理厂的不同生物处理单元（厌氧、好氧等）采集了冬季样本。通过气相色谱-质谱联用技术和高通量测序技术分别鉴定了进水有机成分和活性污泥微生物群落的差异。研究发现，样品的液氮研磨预处理有助于获得更多生物多样性的样本。进水类型和溶解氧浓度影响活性污泥微生物群落结构。硝化螺旋菌科、Caldilineaceae 和 Anaerolineaceae 与生活污水处理系统高度相关，而脱硫微菌属是在工业污水处理系统中发现的最丰富的潜在难降解芳香烃分解菌。在进水成分中，我们推测脱硫微菌属、Macellibacteroides 和脱硫弧菌属是纺织印染工业废水处理系统厌氧环境的关键功能属，而脱硫菌属和硫杆菌属是精细化工废水处理系统的关键功能菌。
