Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, 510260, China.
Sci Rep. 2017 Dec 20;7(1):17928. doi: 10.1038/s41598-017-17915-9.
Trichogramma dendrolimi which is an economically important biological control agent were reared for 30 generations on a modified artificial medium and natural host. Biological characters were assessed and compared with parasitoids reared in vivo. Pupation rate and normal adults rate of in vitro-reared parasitoids were significantly higher compared with in vivo-reared parasitoids. The adult emergence rate, number of adults produced, and fecundity of T. dendrolimi reared in vitro were lower than those reared in vivo. The percentage of females and longevity did not vary between the two rearing methods. The overall fitness of the parasitoids reared artificially from the first to the 20th generation was higher than of those reared from the 21st generation onwards. No differences were observed in the fitness parameters of parasitoids reared in vivo across any of the 30 generations. The results suggest that the modified artificial medium used in this study is suitable for the continuous rearing of T. dendrolimi for at least 20 generations, and has the potential for the mass production of these parasitoids for use in biological control. Such a substrate could be examined for use in rearing other parasitoid species that are important in biological control.
松毛虫赤眼蜂是一种重要的经济生物防治剂，在改良的人工培养基和天然寄主上连续饲养了 30 代。评估了其生物学特性，并与体内饲养的寄生蜂进行了比较。与体内饲养的寄生蜂相比，体外饲养的寄生蜂的化蛹率和正常成虫率显著更高。体外饲养的赤眼蜂的成虫羽化率、成虫数量和产卵量均低于体内饲养的。体外和体内饲养的赤眼蜂的雌蜂比例和寿命没有差异。从第一代到第二十代人工饲养的寄生蜂的整体适应度高于从第二十一代开始饲养的寄生蜂。在任何 30 代中，体内饲养的寄生蜂的适应度参数都没有差异。结果表明，本研究中使用的改良人工培养基适合松毛虫赤眼蜂连续饲养至少 20 代，并且有潜力用于大规模生产这些寄生蜂用于生物防治。可以研究这种基质是否可用于饲养其他在生物防治中很重要的寄生蜂物种。
