Guangdong Entomological Institute, Guangzhou, China.
Insect Sci. 2013 Oct;20(5):629-36. doi: 10.1111/j.1744-7917.2012.01566.x. Epub 2012 Nov 6.
Insect additives have been shown to improve the value of artificial media for Trichogramma species, but at the same time maintain dependence on parallel cultures of host insects. In the present study, Trichogramma dendrolimi Matsumura was reared in vitro from egg to adult on artificial media with different contents of pupal hemolymph of Chinese oak silkmoth Antheraea pernyi (Guérin-Méneville) and with supplements of distilled water, or of trehalose dissolved in water or in Grace's insect medium. The results indicated insect hemolymph was the key component of artificial medium. Developmental parameters, including rates of parasitism, pupation, adult emergence and normal adults, and numbers of produced adults, were increased on media supplemented with trehalose even when the proportion of pupal hemolymph was reduced. Two artificial media, the first containing 30% hemolymph and 10% trehalose in water with 98.9% parasitism rate, 77.7.0% pupation rate, 77.2% emergence rate, 80.0% normal adult rate and 333 produced adults, and the second containing 25% hemolymph and 15% trehalose in Grace's insect medium with 97.8% parasitism rate, 91.0% pupation rate, 85.2% emergence rate, 76.1% normal adult rate and 757 produced adults, were believed to hold potential to mass produce T. dendrolimi. The use of trehalose to partially replace pupal hemolymph in artificial medium of this and other Trichogramma species may contribute to a significant reduction in their production cost and may as such help to evade problems related to short supplies of lepidopteran eggs, which currently constitute the main factitious host for the mass rearing of the parasitoids.
昆虫添加剂已被证明可以提高人工培养基对赤眼蜂属物种的价值，但同时也保持对宿主昆虫平行培养的依赖。在本研究中，松毛虫赤眼蜂从卵到成虫在含有不同浓度的中国栎蚕蛹血淋巴的人工培养基上进行体外培养，并用蒸馏水或溶于水或 Grace 昆虫培养基中的海藻糖进行补充。结果表明，昆虫血淋巴是人工培养基的关键成分。即使降低蛹血淋巴的比例，添加海藻糖也能提高寄生率、化蛹率、成虫羽化率和正常成虫率以及成虫数量等发育参数。两种人工培养基，第一种含有 30%血淋巴和 10%海藻糖在水中，寄生率为 98.9%，化蛹率为 77.7.0%，羽化率为 77.2%，正常成虫率为 80.0%，产生成虫 333 只，第二种含有 25%血淋巴和 15%海藻糖在 Grace 昆虫培养基中，寄生率为 97.8%，化蛹率为 91.0%，羽化率为 85.2%，正常成虫率为 76.1%，产生成虫 757 只，被认为具有大规模生产松毛虫赤眼蜂的潜力。在这种和其他赤眼蜂属物种的人工培养基中用海藻糖部分替代蛹血淋巴，可能有助于显著降低其生产成本，并有助于避免与鳞翅目昆虫卵供应短缺相关的问题，目前这些卵是这些寄生蜂大规模繁殖的主要人工寄主。
