Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China.
School of Life Sciences, Guangzhou University, Guangzhou, China.
Pest Manag Sci. 2019 May;75(5):1328-1338. doi: 10.1002/ps.5247. Epub 2018 Dec 4.
Trichogramma dendrolimi Matsumura is an important egg parasitoid that can be used in the biological control of lepidopteran pests. The effects of storage temperature, developmental stage and storage duration on development and survival were investigated to determine the optimum storage conditions for rearing T. dendrolimi in vitro.
The three investigated factors significantly impacted all the biological parameters except for parental G1 and offspring G2 sex ratios. The responses of in vitro reared T. dendrolimi to low temperatures differed depending on developmental stage and storage duration. Comparison of life table parameters under different storage treatments with the control group indicated that storing prepupae at 13 °C for 2, 3 or 4 weeks positively impacted the growth capacity of the parasitoid.
In vitro reared T. dendrolimi can be reared following cold storage. The quality of the parasitoid was strongly affected by cold storage, likely due to the negative effects of storage on the artificial medium. Storage of the prepupal stage at 13 °C for 4 weeks is optimum for in vitro reared T. dendrolimi. Trichogramma reared in vitro were able to parasitize natural eggs after storage and, thus, have potential for use as biological control agents in the field. © 2018 Society of Chemical Industry.
松毛虫赤眼蜂是一种重要的卵寄生蜂，可用于鳞翅目害虫的生物防治。本研究调查了贮藏温度、发育阶段和贮藏时间对其发育和存活的影响，以确定体外培养松毛虫赤眼蜂的最佳贮藏条件。
这三个因素显著影响了除亲代 G1 和子代 G2 性别比例以外的所有生物学参数。体外饲养的赤眼蜂对低温的反应因发育阶段和贮藏时间而异。不同贮藏处理与对照组的生命表参数比较表明，将预蛹在 13°C 下贮藏 2、3 或 4 周可显著提高寄生蜂的生长能力。
体外培养的赤眼蜂可以进行冷藏后饲养。冷藏对赤眼蜂的质量有很大的影响，可能是由于冷藏对人工培养基的负面影响。在 13°C 下贮藏预蛹 4 周是体外培养赤眼蜂的最佳条件。冷藏后的赤眼蜂能够寄生自然卵，因此具有在田间作为生物防治剂的潜力。© 2018 英国化学学会。
