Kumar Niranjan, Das Bhupamani, Solanki Jayesh B, Jadav Mehul M, Menaka Ramasamy
Department of Parasitology, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari - 396 450, Gujarat, India.
Department of Anatomy, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari-396 450, Gujarat, India.
Vet World. 2017 Nov;10(11):1394-1400. doi: 10.14202/vetworld.2017.1394-1400. Epub 2017 Nov 29.
Preservation of macroparasites by infiltrating the polymer in the tissues can defy the inherited shortcoming of classical wet preservation method.
Preservation was done by infiltrating the melamine alone or with xylene (MX)/chloroform (MC)/turpentine oil (MT) in 1:1 and hardener (MH) in 9:1 ratio in the tissues of the gross specimen of the animal parasites.
The plastinated models withstand the process of microbial decomposition, and remain intact in the environmental conditions. The polymer mixture resists the entry of the water molecule, and model dried just after taking out it from the water tank. Overall, the plastinated parasites were dry, non-sticky, glossy, odorless, chemical free, and harmless, to some extent flexible, with detectable morphological structure, and retain their natural form but lost their natural color. Full marks were assigned to the degree of dryness, non-stickiness, and odorlessness to the model plastinated in different solutions on a five-point scale. For flexibility, the score was 1.2, 2.2, and 2.4 for the plastinated model in melamine/MH, MX/MC, and MT solutions, respectively. The average score of glossiness was 4.6 and 5 for the specimen plastinated in melamine/MH and MX/MC/MT solutions, respectively. The degree of dryness, glossiness, stickiness, and flexibility varies non-significantly, with the polymer mixtures used.
The prepared model can be used to educate the students/general mass population.
通过在组织中渗透聚合物来保存大型寄生虫，可以克服传统湿保存方法固有的缺点。
在动物寄生虫大体标本的组织中，通过单独渗透三聚氰胺或与二甲苯（MX）/氯仿（MC）/松节油（MT）按1:1比例以及硬化剂（MH）按9:1比例进行保存。
塑化模型能够经受微生物分解过程，并在环境条件下保持完整。聚合物混合物能阻止水分子进入，模型从水箱中取出后就会干燥。总体而言，塑化后的寄生虫干燥、不粘、有光泽、无气味、无化学物质且无害，在一定程度上具有柔韧性，具有可检测的形态结构，保留其自然形态但失去了自然颜色。在五点量表上，对不同溶液中塑化模型的干燥程度、不粘性和无气味程度给予满分。对于柔韧性，三聚氰胺/MH、MX/MC和MT溶液中塑化模型的得分分别为1.2、2.2和2.4。三聚氰胺/MH和MX/MC/MT溶液中塑化标本的光泽度平均得分分别为4.6和5。干燥程度、光泽度、粘性和柔韧性随所用聚合物混合物的不同而变化不显著。
制备的模型可用于教育学生/普通大众。
