CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.
Appl Microbiol Biotechnol. 2018 Feb;102(4):1535-1544. doi: 10.1007/s00253-017-8695-5. Epub 2017 Dec 20.
Sabinene is an important naturally occurring bicyclic monoterpene which can be used as flavorings, perfume additives, fine chemicals, and advanced biofuels. Up to now, this valuable terpene is commercially unavailable since there is no applicable manufacturing process. Microbial synthesis can be a promising route for sabinene production. In this review, we summarize knowledge about the metabolic pathway and key enzymes for sabinene biosynthesis. Recent advances that have been made in production of sabinene by microbial fermentation are highlighted. In these studies, researchers have identified the general synthetic pathway of sabinene from simple intermediate metabolites. Sabinene synthases of different origins were also cloned and characterized. Additionally, heterologous systems of the model microbes Escherichia coli and Saccharomyces cerevisiae were constructed to produce sabinene. This review also suggests new directions and attempts to gain some insights for achieving an industrial level production of sabinene. The combination of traditional molecular biology with new genome and proteome analysis tools will provide a better view of sabinene biosynthesis and a greater potential of microbial production.
柠檬烯是一种重要的天然双环单萜，可用作香料、香水添加剂、精细化学品和先进的生物燃料。到目前为止，由于没有适用的制造工艺，这种有价值的萜烯在商业上无法获得。微生物合成可能是生产柠檬烯的一种有前途的途径。在这篇综述中，我们总结了柠檬烯生物合成的代谢途径和关键酶的知识。重点介绍了通过微生物发酵生产柠檬烯的最新进展。在这些研究中，研究人员已经确定了从简单的中间代谢物合成柠檬烯的一般途径。还克隆和表征了不同来源的柠檬烯合酶。此外，构建了模型微生物大肠杆菌和酿酒酵母的异源系统来生产柠檬烯。本综述还提出了实现柠檬烯工业生产的新方向和尝试，为实现工业水平的生产提供了一些见解。传统分子生物学与新的基因组和蛋白质组分析工具的结合将为柠檬烯生物合成提供更好的视角，并为微生物生产提供更大的潜力。
