Department of Laboratory Medicine and School of Public Health, Jilin Medical University, Jilin, Jilin, China.
Affiliated Hospital of Jilin Medical University, Jilin, Jilin, China.
Biochem Biophys Res Commun. 2022 Jun 30;611:140-145. doi: 10.1016/j.bbrc.2022.04.049. Epub 2022 Apr 26.
Osteoblasts in multicellular organisms are sensitive to fluid shear stress (Fss) and respond smartly with versatile patterns of intracellular calcium signal ([Ca]). In this study, a spatial-single cell patterning method was developed by combining micro-contact printing (μCP) and reversible microfluidic chip mounted with vacuum together. Based on this well-defined patterning platform, it's possible to investigate calcium response to Fss modulated by spatial factors, and to characterize multiple calcium patterns quantitatively in terms of cell spacing and cell orientation. The result showed that the Fss-induced [Ca] profiles revealed oscillational signal patterns in non-connected cells such as those in physical-contacted cells. Close-arrayed osteoblasts showed remarkably more [Ca] oscillations than sparse-arrayed cells. The circular shape of the cells was sensitive to oscillational [Ca] as a potential major cause. The consistency of cell orientation and shear stress promoted temporal homogeneity of calcium oscillations.
多细胞生物中的成骨细胞对流体切应力 (Fss) 敏感，并通过多种细胞内钙信号 ([Ca]) 模式做出灵敏反应。在这项研究中，通过结合微接触印刷 (μCP) 和带有真空的可逆微流控芯片，开发了一种空间单细胞图案化方法。基于这个精确定义的图案化平台，可以研究由空间因素调制的 Fss 对钙的响应，并从细胞间距和细胞取向的角度对多种钙图案进行定量表征。结果表明，Fss 诱导的 [Ca] 图谱揭示了非接触细胞（如物理接触细胞）中呈现的振荡信号模式。紧密排列的成骨细胞比稀疏排列的细胞表现出更明显的 [Ca] 振荡。细胞的圆形形状对振荡 [Ca] 很敏感，是潜在的主要原因。细胞取向和切应力的一致性促进了钙振荡的时间均匀性。
