Department of Stomatology, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China.
Department of Anatomy and Cell Biology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Stem Cells. 2018 Apr;36(4):551-561. doi: 10.1002/stem.2762. Epub 2018 Jan 4.
Oral microbiome is essential for maintenance of oral cavity health. Imbalanced oral microbiome causes periodontal and other diseases. It is unknown whether oral microbiome affect oral stem cells function. This study used a common clinical antibiotic treatment approach to alter oral microbiome ecology and examine whether oral mesenchymal stem cells (MSCs) are affected. We found that altered oral microbiome resulted gingival MSCs deficiency, leading to a delayed wound healing in male mice. Mechanistically, oral microbiome release lipopolysaccharide (LPS) that stimulates the expression of microRNA-21 (miR-21) and then impair the normal function of gingival MSCs and wound healing process through miR-21/Sp1/telomerase reverse transcriptase pathway. This is the first study indicate that interplay between oral microbiome and MSCs homeostasis in male mice. Stem Cells 2018;36:551-561.
口腔微生物组对于维持口腔健康至关重要。口腔微生物组失衡会导致牙周病等疾病。目前尚不清楚口腔微生物组是否会影响口腔干细胞的功能。本研究采用常见的临床抗生素治疗方法来改变口腔微生物组生态，并观察口腔间充质干细胞（MSCs）是否受到影响。我们发现，口腔微生物组的改变导致牙龈 MSCs 缺失，从而导致雄性小鼠伤口愈合延迟。在机制上，口腔微生物组释放脂多糖（LPS），刺激 microRNA-21（miR-21）的表达，然后通过 miR-21/Sp1/端粒酶逆转录酶通路损害牙龈 MSCs 的正常功能和伤口愈合过程。这是第一项研究表明，口腔微生物组与雄性小鼠间充质干细胞稳态之间存在相互作用。《干细胞》2018 年；36:551-561。
