Santos Carolyne D S, Balbo Sandra L, Guimarães Ana T B, Sagae Sara C, Negretti Fábio, Grassiolli Sabrina
Laboratório de Fisiologia Endócrina e Metabolismo/LAFEM, Centro de Ciências Biológicas e da Saúde/CCBS, Universidade Estadual do Oeste do Paraná/UNIOESTE, Rua Universitária, 2069, Jardim Universitário, 85819-110 Cascavel, PR, Brazil.
Laboratório de Fisiologia Endócrina e Metabolismo/LAFEM, Centro de Ciências Médicas e Farmacêuticas/CCMF, Universidade Estadual do Oeste do Paraná/UNIOESTE, Rua Universitária, 2069, Jardim Universitário, 85819-110 Cascavel, PR, Brazil.
An Acad Bras Cienc. 2017 Oct-Dec;89(4):2887-2900. doi: 10.1590/0001-3765201720170316.
Here, we evaluated whether the exposure of rats to a cafeteria diet pre- and/or post-weaning, alters histological characteristics in the White Adipose Tissue (WAT), Brown Adipose Tissue (BAT), and liver of adult male offspring. Female Wistar rats were divided into Control (CTL; fed on standard rodent chow) and Cafeteria (CAF; fed with the cafeteria diet throughout life, including pregnancy and lactation). After birth, only male offspring (F1) were maintained and received the CTL or CAF diets; originating four experimental groups: CTL-CTLF1; CTL-CAFF1; CAF-CTLF1; CAF-CAFF1. Data of biometrics, metabolic parameters, liver, BAT and WAT histology were assessed and integrated using the Principal Component Analysis (PCA). According to PCA analysis worse metabolic and biometric characteristics in adulthood are associated with the post-weaning CAF diet compared to pre and post weaning CAF diet. Thus, the CTL-CAFF1 group showed obesity, higher deposition of fat in the liver and BAT and high fasting plasma levels of glucose, triglycerides and cholesterol. Interestingly, the association between pre and post-weaning CAF diet attenuated the obesity and improved the plasma levels of glucose and triglycerides compared to CTL-CAFF1 without avoiding the higher lipid accumulation in BAT and in liver, suggesting that the impact of maternal CAF diet is tissue-specific.
在此，我们评估了大鼠在断奶前和/或断奶后食用自助式饮食，是否会改变成年雄性后代白色脂肪组织（WAT）、棕色脂肪组织（BAT）和肝脏的组织学特征。雌性Wistar大鼠被分为对照组（CTL；喂食标准啮齿动物饲料）和自助式饮食组（CAF；终生喂食自助式饮食，包括怀孕和哺乳期）。出生后，仅保留雄性后代（F1）并给予CTL或CAF饮食；由此产生四个实验组：CTL-CTLF1；CTL-CAFF1；CAF-CTLF1；CAF-CAFF1。使用主成分分析（PCA）对生物统计学、代谢参数、肝脏、BAT和WAT组织学数据进行评估和整合。根据PCA分析，与断奶前和断奶后均食用CAF饮食相比，成年后较差的代谢和生物统计学特征与断奶后食用CAF饮食有关。因此，CTL-CAFF1组表现出肥胖、肝脏和BAT中脂肪沉积增加以及空腹血浆葡萄糖、甘油三酯和胆固醇水平升高。有趣的是，与CTL-CAFF1组相比，断奶前和断奶后食用CAF饮食之间的关联减轻了肥胖，并改善了血浆葡萄糖和甘油三酯水平，尽管未能避免BAT和肝脏中较高的脂质积累，这表明母体CAF饮食的影响具有组织特异性。
