Graduate School of Horticulture, Chiba University, Matsudo 271-8510, Japan.
Graduate School of Horticulture, Chiba University, Matsudo 271-8510, Japan; Center for Environment, Health and Field Sciences, Chiba University, Kashiwa-no-ha 277-0882, Japan.
J Plant Physiol. 2018 Feb;221:85-93. doi: 10.1016/j.jplph.2017.12.007. Epub 2017 Dec 16.
The production of aromatic volatiles such as esters during the ripening process in climacteric fruits is known to be controlled by ethylene. However, we here show that abscisic acid (ABA) application accelerated the onset of short-chain ester production (hexyl propionate, ethyl-2-methyl butyrate) and the expression of biosynthesis genes (MdAAT2 and MdBCAT1) during ripening of 'Orin' apple. ABA application also promoted the production of ethylene, and caused ethylene peak shifts correlated with the expression of ethylene synthesis genes (MdACS1/3 and MdACO1), suggesting that ABA may act jointly with ethylene as a positive regulator at the ripening stage of 'Orin' apple. Additionally, endogenous levels and expression of biosynthesis (MdNCED1) and signal transduction genes (MdABF2-like) of ABA increased towards ripening. Finally, the localization of the putative MdABF2-like protein binding element, AREB/ABF, was observed in the 5'-upstream region of MdACS1/3 and MdACO1.
在跃变型果实的成熟过程中，芳香挥发性物质（如酯类）的产生被认为受乙烯调控。然而，我们在这里表明，脱落酸（ABA）的应用加速了‘Orin’苹果成熟过程中短链酯（己酸丙酯、乙基-2-甲基丁酸酯）的产生和生物合成基因（MdAAT2 和 MdBCAT1）的表达。ABA 的应用也促进了乙烯的产生，并导致与乙烯合成基因（MdACS1/3 和 MdACO1）表达相关的乙烯峰位移动，这表明 ABA 可能与乙烯一起作为‘Orin’苹果成熟阶段的正调控因子发挥作用。此外，ABA 的生物合成（MdNCED1）和信号转导基因（MdABF2-like）的内源性水平和表达在成熟过程中增加。最后，观察到假定的 MdABF2-like 蛋白结合元件 AREB/ABF 位于 MdACS1/3 和 MdACO1 的 5'-上游区。
