Malhotra S K, Predy R, Johnson E S, Singh R, Leeuw K
Department of Zoology, University of Alberta, Edmonton, Canada.
J Neurosci Res. 1989 Jan;22(1):36-49. doi: 10.1002/jnr.490220106.
Monoclonal antibody J1-31 (MAb J1-31, isotype IgG 2b) was raised against crude homogenate of brain tissue from a multiple sclerosis (MS) patient (autopsy sample; Malhotra et al.: Microbios Letters 26:151-157, 1984). In human brain, MAb J1-31 recognizes an intracellular protein antigen (J1-31 antigen), which bands at approximately 30,000 daltons under reducing conditions for sodium dodecyl sulfate gel electrophoresis (Singh et al.: Bioscience Reports 6:73-79, 1986). By immunofluorescence microscopy, MAb J1-31 stains those cells that are also stained by antiserum to glial fibrillary acidic protein (GFAP), namely astrocytes, retinal Müller cells, and tanycytes in the ependyma (Predy et al.: Bioscience Reports 7:491-502, 1987). In addition, MAb J1-31 stains ciliated ependymal cells that do not express GFAP. Using a model system for gliosis (laceration-type injury of rat spinal cord), we were able to show that astrocytes responding to central nervous system injury exhibit greatly enhanced staining for J1-31 antigen (Predy et al.: Journal of Neuroscience Research 19:397-404, 1988; Predy and Malhotra: Brain Research Bulletin in press, 1989). In this article, we demonstrate that immunofluorescence staining owing to MAb J1-31 is greatly enhanced in MS plaques, as compared to adjacent "apparently normal" white matter. (This is consistent with previous results as MS plaques characteristically show an astroglial response [reactive gliosis] leading to the formation of a glial scar [McKhann: Annual Review of Neuroscience 5:219-239, 1982].) In addition, we present further evidence that J1-31 antigen is distinct from GFAP, although these two proteins may be associated spatially with one another.
单克隆抗体J1 - 31（MAb J1 - 31，同种型IgG 2b）是针对一名多发性硬化症（MS）患者（尸检样本；Malhotra等人：《微生物学快报》26:151 - 157，1984年）脑组织的粗匀浆产生的。在人脑中，MAb J1 - 31识别一种细胞内蛋白质抗原（J1 - 31抗原），在十二烷基硫酸钠凝胶电泳的还原条件下，该抗原的条带约为30,000道尔顿（Singh等人：《生物科学报告》6:73 - 79，1986年）。通过免疫荧光显微镜检查，MAb J1 - 31对那些也被抗胶质纤维酸性蛋白（GFAP）抗血清染色的细胞进行染色，即星形胶质细胞、视网膜穆勒细胞和室管膜中的室管膜细胞（Predy等人：《生物科学报告》7:491 - 502，1987年）。此外，MAb J1 - 31对不表达GFAP的纤毛室管膜细胞进行染色。使用胶质增生的模型系统（大鼠脊髓撕裂伤型损伤），我们能够证明对中枢神经系统损伤作出反应的星形胶质细胞对J1 - 31抗原的染色大大增强（Predy等人：《神经科学研究杂志》19:397 - 404，1988年；Predy和Malhotra：《脑研究通报》待发表，1989年）。在本文中，我们证明与相邻的“看似正常”白质相比，MS斑块中由于MAb J1 - 31引起的免疫荧光染色大大增强。（这与先前的结果一致，因为MS斑块典型地显示星形胶质细胞反应[反应性胶质增生]导致胶质瘢痕的形成[McKhann：《神经科学年度评论》5:219 - 239，1982年]。）此外，我们提供了进一步的证据表明J1 - 31抗原与GFAP不同，尽管这两种蛋白质可能在空间上相互关联。
