Bhatnagar R, Malhotra S K
Department of Biological Sciences, University of Alberta, Edmonton, Canada.
Cytobios. 1995;82(331):239-49.
Several methods for the detection of immunogold labelling for the monoclonal antibody (Mab) J1-31 in astroglial cells are described. The optimal preservation of the gold labelling is achieved in sections after cryoultramicrotomy or LR white embedding. The results obtained are consistent with the previous findings that the Mab J1-31 antigen is an intermediate filament-associated protein in the astroglial cells.
本文描述了几种检测星形胶质细胞中单克隆抗体（Mab）J1-31免疫金标记的方法。在冷冻超薄切片或LR白色包埋后的切片中，可实现金标记的最佳保存。所得结果与先前的发现一致，即Mab J1-31抗原是星形胶质细胞中的一种中间丝相关蛋白。
