Predy R, Malhotra S K, Das G D
Department of Pathology, University of Alberta, Edmonton, Canada.
J Neurosci Res. 1988 Apr;19(4):397-404, 466-7. doi: 10.1002/jnr.490190403.
A mouse monoclonal antibody (MAb J1-31, isotype IgG 2b) was raised against an autopsy sample of cerebral white matter from a multiple sclerosis (MS) patient. MAb J1-31 recognizes a protein (J1-31 antigen) in human brain which has a molecular weight of approximately 30,000 daltons (30 kD) as determined by immunoprecipitation followed by SDS-gel electrophoresis (reducing conditions) and autoradiography (Singh et al.: Biosci Rep 6:73-79, 1986). By immunofluorescence microscopy, MAb J1-31 stains glial fibrillary acidic protein (GFAP)-positive cells, namely astrocytes, of both human and rat. Yet J1-31 antigen is distinct from GFAP (Predy et al.: Biosci Rep 7:491-502, 1987). In this paper we report that greatly enhanced staining for J1-31 antigen is exhibited by reactive astrocytes which arise following CNS injury. (Laceration-type surgical lesion of the rat spinal cord served as the experimental model). Enhanced expression of J1-31 antigen reveals some new aspect of the astrocyte response to injury, involving transformation to the reactive state. Consequently, MAb J1-31 may be a useful marker for studies on reactive astrocytes.
针对一名多发性硬化症（MS）患者脑白质的尸检样本制备了一种小鼠单克隆抗体（MAb J1-31，同型IgG 2b）。MAb J1-31识别出人类大脑中的一种蛋白质（J1-31抗原），通过免疫沉淀、随后的SDS凝胶电泳（还原条件）和放射自显影法测定，其分子量约为30,000道尔顿（30 kD）（Singh等人：《生物科学报告》6：73-79，1986年）。通过免疫荧光显微镜观察，MAb J1-31可对人类和大鼠的胶质纤维酸性蛋白（GFAP）阳性细胞（即星形胶质细胞）进行染色。然而，J1-31抗原与GFAP不同（Predy等人：《生物科学报告》7：491-502，1987年）。在本文中，我们报告了中枢神经系统损伤后出现的反应性星形胶质细胞对J1-31抗原的染色大大增强。（大鼠脊髓的撕裂型手术损伤用作实验模型）。J1-31抗原表达的增强揭示了星形胶质细胞对损伤反应的一些新方面，涉及向反应性状态的转变。因此，MAb J1-31可能是研究反应性星形胶质细胞的有用标志物。
