Chu Zhaoxia, Wang Xingming, Wang Yunmin, Liu Guijian, Dong Zhongbing, Lu Xianwen, Chen Guangzhou, Zha Fugeng
School of Earth and Environment, Anhui University of Science and Technology, Huainan, 232001, China.
School of Bioengineering, Huainan Normal University, Huainan, 232038, China.
Environ Monit Assess. 2017 Dec 21;190(1):36. doi: 10.1007/s10661-017-6400-x.
Copper mine tailings pose many threats to the surrounding environment and human health, and thus, their remediation is fundamental. Coal spoil is the waste by-product of coal mining and characterized by low levels of metals, high content of organic matter, and many essential microelements. This study was designed to evaluate the role of coal spoil on heavy uptake and physiological responses of Lolium perenne L. grown in copper mine tailings amended with coal spoil at rates of 0, 0.5, 1, 5, 10, and 20%. The results showed that applying coal spoil to copper mine tailings decreased the diethylenetriaminepentaacetic acid (DTPA)-extractable Cd, Cu, Pb, and Zn contents in tailings and reduced those metal contents in both roots and shoots of the plant. However, application of coal spoil increased the DTPA-extractable Cr concentration in tailings and also increased Cr uptake and accumulation by Lolium perenne L. The statistical analysis of physiological parameters indicated that chlorophyll and carotenoid increased at the lower amendments of coal spoil followed by a decrease compared to their respective controls. Protein content was enhanced at all the coal spoil amendments. When treated with coal spoil, the activities of superoxide dismutases (SOD), peroxidase (POD), and catalase (CAT) responded differently. CAT activity was inhibited, but POD activity was increased with increasing amendment ratio of coal spoil. SOD activity increased up to 1% coal spoil followed by a decrease. Overall, the addition of coal spoil decreased the oxidative stress in Lolium perenne L., reflected by the reduction in malondialdehyde (MDA) contents in the plant. It is concluded that coal spoil has the potential to stabilize most metals studied in copper mine tailings and ameliorate the harmful effects in Lolium perenne L. through changing the physiological attributes of the plant grown in copper mine tailings.
铜矿尾矿对周边环境和人类健康构成诸多威胁，因此对其进行修复至关重要。煤矸石是煤炭开采的废弃物副产品，其特点是金属含量低、有机质含量高且含有多种必需微量元素。本研究旨在评估煤矸石对生长在添加了煤矸石的铜矿尾矿中的多年生黑麦草重金属吸收及生理响应的作用，煤矸石添加比例分别为0%、0.5%、1%、5%、10%和20%。结果表明，向铜矿尾矿中添加煤矸石降低了尾矿中可被二乙烯三胺五乙酸（DTPA）提取的镉、铜、铅和锌含量，并减少了植物根和地上部分的这些金属含量。然而，添加煤矸石增加了尾矿中可被DTPA提取的铬浓度，也增加了多年生黑麦草对铬的吸收和积累。生理参数的统计分析表明，在较低煤矸石添加量时叶绿素和类胡萝卜素含量增加，随后与各自对照相比有所下降。在所有煤矸石添加处理下蛋白质含量均有所提高。用煤矸石处理时，超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的活性表现不同。CAT活性受到抑制，但POD活性随煤矸石添加比例的增加而升高。SOD活性在煤矸石添加量达1%时升高，随后下降。总体而言，煤矸石的添加降低了多年生黑麦草的氧化应激，这体现在植物中丙二醛（MDA）含量的降低上。结论是，煤矸石有潜力稳定铜矿尾矿中所研究的大多数金属，并通过改变生长在铜矿尾矿中的植物的生理特性来减轻对多年生黑麦草的有害影响。
