Fujimoto Haruhiro
, Chiba, Japan.
J Nat Med. 2018 Jan;72(1):20-31. doi: 10.1007/s11418-017-1162-x. Epub 2017 Dec 21.
Our screening project, namely, search for new immunomodulatory constituents from Ascomycetous fungi, was guided by the effects on mitogen-induced proliferations of mouse spleen lymphocytes. On the project, the defatted crude extracts from Gelasinospora multiforis, G. heterospora, G. longispora, G. kobi, Diplogelasinospora grovesii, Emericella aurantio-brunnea, Eupenicillium crustaceum, etc., submitted to the solvent partition followed by fractionation with repeated chromatography monitored by immunomodulatory activity to afford many active constituents, of which molecular structures including absolute configurations and immunomodulatory activities were elucidated. All of these immunomodulatory constituents isolated on the project were practically not immunostimulants but immunosuppressants.
我们的筛选项目，即从子囊菌中寻找新的免疫调节成分，是受其对小鼠脾淋巴细胞有丝分裂原诱导增殖的影响所引导。在该项目中，对多孢胶枝霉、异孢胶枝霉、长孢胶枝霉、科比胶枝霉、格氏双胶枝霉、金棕曲霉、甲壳青霉等的脱脂粗提物进行溶剂分配，然后通过免疫调节活性监测进行反复色谱分离以获得许多活性成分，其中包括绝对构型的分子结构和免疫调节活性都得到了阐明。该项目中分离出的所有这些免疫调节成分实际上都不是免疫刺激剂而是免疫抑制剂。
