青春期前肥胖儿童循环中miR-122水平与非酒精性脂肪性肝病
Circulating levels of miR-122 and nonalcoholic fatty liver disease in pre-pubertal obese children.
作者信息
Brandt S, Roos J, Inzaghi E, Kotnik P, Kovac J, Battelino T, Cianfarani S, Nobili V, Colajacomo M, Kratzer W, Denzer C, Fischer-Posovszky P, Wabitsch M
机构信息
Division of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, University Medical Center Ulm, Ulm, Germany.
Dipartimento Pediatrico Universitario Ospedaliero, Bambino Gesù Children's Hospital, Tor Vergata University, Rome, Italy.
出版信息
Pediatr Obes. 2018 Mar;13(3):175-182. doi: 10.1111/ijpo.12261. Epub 2017 Dec 22.
OBJECTIVES
The liver-specific miR-122 was proposed as biomarker for NAFLD in adults. Here, we investigated the relationship between miR-122 levels, parameters of liver metabolism and NAFLD in pre-pubertal obese children.
METHODS
Parameters of liver metabolism (ALT, AST and GGT) of three European cohorts were included (German cohort [n = 71; age: 11.53 ± 1.29 years; BMI z-score: 2.96 ± 0.64], Italian cohort [n = 45; age: 9.60 ± 2.11 years; BMI z-score: 3.57 ± 1.16], Slovenian cohort [n = 31; age: 7.53 ± 1.47 years; BMI z-score: 3.66 ± 0.88]). MiR-122 levels and CK18 concentrations were measured in fasting blood samples. In the German and Italian cohort, the diagnosis of NAFLD and grading of NAFLD was assessed by ultrasound.
RESULTS
NAFLD was diagnosed in n = 50 patients of the German cohort (29.6%) and in n = 29 patients (72.5%) of the Italian cohort. In all three cohorts, miR-122 was positively correlated with ALT and AST as well as with CK18 concentrations. MiR-122 levels were higher in children with NAFLD compared with healthy controls.
CONCLUSIONS
MiR-122 levels in pre-pubertal obese children could be a potential biomarker for paediatric NAFLD.
目的
肝脏特异性微小RNA-122（miR-122）被认为是成人非酒精性脂肪性肝病（NAFLD）的生物标志物。在此，我们研究了青春期前肥胖儿童中miR-122水平、肝脏代谢参数与NAFLD之间的关系。
方法
纳入三个欧洲队列的肝脏代谢参数（谷丙转氨酶、谷草转氨酶和γ-谷氨酰转肽酶）（德国队列[n = 71；年龄：11.53±1.29岁；体重指数z评分：2.96±0.64]，意大利队列[n = 45；年龄：9.60±2.11岁；体重指数z评分：3.57±1.16]，斯洛文尼亚队列[n = 31；年龄：7.53±1.47岁；体重指数z评分：3.66±0.88]）。在空腹血样中测量miR-122水平和细胞角蛋白18（CK18）浓度。在德国和意大利队列中，通过超声评估NAFLD的诊断和NAFLD分级。
结果
德国队列中50例患者（29.6%）和意大利队列中29例患者（72.5%）被诊断为NAFLD。在所有三个队列中，miR-122与谷丙转氨酶、谷草转氨酶以及CK18浓度呈正相关。与健康对照相比，NAFLD儿童的miR-122水平更高。
结论
青春期前肥胖儿童的miR-122水平可能是儿童NAFLD的潜在生物标志物。
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