Department of Gastroenterology, Affiliated Wujiang Hospital of Nantong University, Suzhou, China.
Eur Rev Med Pharmacol Sci. 2017 Dec;21(24):5661-5667. doi: 10.26355/eurrev_201712_14010.
To investigate the expression of long non-coding RNA CASC15 in gastric cancer tissue and its effect on the proliferation of gastric cancer cell line MKN28.
We found that expression of lncRNA CASC15 in gastric cancer tissue was higher than normal gastric epithelium through the TCGA and Gene Expression Omnibus (GEO) database. Then, we detect the RNA level of CASC15 from clinical samples of 42 normal gastric epithelial tissues and 60 gastric cancer tissues. In order to explore the function of CASC15 in gastric cancer, we perform gain-function and loss-function assay in gastric cancer cell lines.
We found that expression of lncRNA CASC15 in gastric cancer tissue was higher than normal gastric epithelium through the TCGA database and the related microarray data set was searched from Gene Expression Omnibus (GEO) database. Then, we extracted total RNA from clinical samples of 42 normal gastric epithelial tissues and 60 gastric cancer tissues. The results of quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) were consistent with those of TCGA analysis. Clinical data analysis showed that the expression of LncRNA CASC15 was correlated with the total survival, tumor size and TMN staging in clinical patients. Clinical data analysis showed that the expression level of CASC15 was correlated with tumor size and TNM stage in clinical patients. Compared with the negative control group, the proliferation and cell cloning ability of MKN28 cells overexpressing LncRNA CASC15 significantly increased (p<0.001), indicating that overexpression of LncRNA CASC15 promoted the proliferation of MKN28 cells.
The expression of LncRNA CASC15 was significantly higher in gastric cancer tissues and its expression was negatively correlated with the overall survival of clinical patients. It was positively correlated with the tumor size and TMN stage. LncRNA CASC15 could promote the proliferation of gastric cancer cells and was expected to become the molecular marker for prediction and prognosis of gastric cancer, as well as a potential therapeutic target.
探讨长链非编码 RNA CASC15 在胃癌组织中的表达及其对胃癌细胞系 MKN28 增殖的影响。
我们通过 TCGA 和基因表达综合数据库（GEO）数据库发现胃癌组织中 lncRNA CASC15 的表达高于正常胃上皮组织。然后，我们检测了 42 例正常胃上皮组织和 60 例胃癌组织临床样本中 CASC15 的 RNA 水平。为了探讨 CASC15 在胃癌中的功能，我们在胃癌细胞系中进行了增益功能和缺失功能实验。
我们通过 TCGA 数据库发现胃癌组织中 lncRNA CASC15 的表达高于正常胃上皮组织，并且从 GEO 数据库中搜索到了相关的微阵列数据集。然后，我们从 42 例正常胃上皮组织和 60 例胃癌组织临床样本中提取总 RNA。定量逆转录-聚合酶链反应（qRT-PCR）的结果与 TCGA 分析一致。临床数据分析表明，LncRNA CASC15 的表达与临床患者的总生存、肿瘤大小和 TMN 分期有关。临床数据分析表明，CASC15 的表达水平与临床患者的肿瘤大小和 TNM 分期有关。与阴性对照组相比，过表达 LncRNA CASC15 的 MKN28 细胞的增殖和细胞克隆能力显著增加（p<0.001），表明 LncRNA CASC15 的过表达促进了 MKN28 细胞的增殖。
LncRNA CASC15 在胃癌组织中的表达明显升高，其表达与临床患者的总生存率呈负相关。与肿瘤大小和 TMN 分期呈正相关。LncRNA CASC15 可促进胃癌细胞的增殖，有望成为预测和预后胃癌的分子标志物，以及潜在的治疗靶点。
