Cook Ryan, Hannon Drew, Southard Jonathan N, Majumdar Sudipta
Department of Chemistry, Indiana University of Pennsylvania, Indiana, Pennsylvania, 15705.
Biochem Mol Biol Educ. 2018 Mar;46(2):172-181. doi: 10.1002/bmb.21102. Epub 2017 Dec 23.
A one semester undergraduate biochemistry laboratory experience is described for an understanding of recombinant technology from gene cloning to protein characterization. An integrated experimental design includes three sequential modules: molecular cloning, protein expression and purification, and protein analysis and characterization. Students perform the tasks of cloning, expression, purification, analysis, and characterization of small laccase (SLAC) from Streptomyces coelicolor. SLAC is an extremely robust well-characterized protein/enzyme, which serves as an ideal model for undergraduate teaching laboratories. Also, this goal-oriented research-like approach helps students to unite the concepts of biochemistry and molecular biology and appreciate the utility of the methods more effectively. A student assessment before and after the course demonstrated an overall increase in learning and enthusiasm on the topic of modern protein chemistry. © 2017 by The International Union of Biochemistry and Molecular Biology, 46(2):172-181, 2018.
本文描述了一个为期一学期的本科生物化学实验室经历，旨在让学生了解从基因克隆到蛋白质表征的重组技术。一个综合实验设计包括三个连续模块：分子克隆、蛋白质表达与纯化，以及蛋白质分析与表征。学生们完成了来自天蓝色链霉菌的小漆酶（SLAC）的克隆、表达、纯化、分析和表征任务。SLAC是一种特性极其明确的稳健蛋白质/酶，是本科教学实验室的理想模型。此外，这种以目标为导向的类似研究的方法有助于学生将生物化学和分子生物学的概念结合起来，并更有效地理解这些方法的实用性。课程前后的学生评估表明，学生在现代蛋白质化学主题上的学习和热情总体有所提高。© 2017年，国际生物化学与分子生物学联盟，46(2):172 - 181，2018年。
