Periyannan Gopal R
Department of Chemistry and Biochemistry, Eastern Illinois University, Charleston, Illinois, 61920.
Biochem Mol Biol Educ. 2019 Jul;47(4):438-445. doi: 10.1002/bmb.21237. Epub 2019 Mar 28.
Technique-centered biochemistry or molecular biology undergraduate laboratory curricula do not offer significant opportunities for thoughtful, in-depth exploration of the science to foster investigative learning. To demonstrate inclusion of inquiry-driven laboratory experiments into the undergraduate biochemistry and molecular biology curricula, a comprehensive set of laboratory experiments, covering several principles of biochemistry and molecular biology, have been developed under a single theme. The laboratory curriculum described here comprehensively investigates bacterial cellobiose metabolism using multiple biochemical, molecular biological (RNA isolation, RT-PCR, PCR, and enzyme assay), and analytical techniques (High Performance Liquid Chromatography, NMR, spectrophotometry, and thin-layer chromatography) to explore the principles of metabolomics and genomics in a single undergraduate laboratory course setting using Caulobacter crescentus as the model organism. This laboratory module serves as a model for educators to develop easy-to-implement laboratory curricula incorporating contemporary biochemistry and molecular biology concepts and techniques to provide a course-based undergraduate research experiences (CUREs) with defined learning objectives. © 2019 International Union of Biochemistry and Molecular Biology, 47(4):438-445, 2019.
以技术为中心的生物化学或分子生物学本科实验室课程，并未提供大量机会让学生深入、有深度地探索科学，以培养探究式学习。为了展示将探究驱动的实验室实验纳入本科生物化学和分子生物学课程，我们围绕一个单一主题开发了一套全面的实验室实验，涵盖生物化学和分子生物学的多个原理。本文所述的实验室课程使用多种生化、分子生物学（RNA分离、逆转录聚合酶链式反应、聚合酶链式反应和酶活性测定）以及分析技术（高效液相色谱法、核磁共振、分光光度法和薄层色谱法），以新月柄杆菌作为模式生物，在单一的本科实验室课程设置中全面研究细菌纤维二糖代谢，探索代谢组学和基因组学原理。这个实验室模块为教育工作者提供了一个范例，用于开发易于实施的实验室课程，融入当代生物化学和分子生物学概念与技术，以提供具有明确学习目标的基于课程的本科研究经历（CUREs）。© 2019国际生物化学与分子生物学联盟，47(4):438 - 445, 2019。
