Ghosh R, Bhattacharjee S B
Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Calcutta, India.
Mutat Res. 1989 Mar;225(3):137-41. doi: 10.1016/0165-7992(89)90131-0.
Density-inhibited V79 cells when held for 24 h in complete medium after exposure to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) show improved survival levels and decreased mutant frequencies at all dose levels, compared to cells not so held. However, when benzamide, an inhibitor of poly(ADP-ribose) synthesis was present during this 24-h holding, the improvement in survival and decrease in mutant frequencies were not observed. Rather, compared to the control, the cells became more sensitive to MNNG and mutant frequency also increased significantly for all doses studied.
暴露于N-甲基-N'-硝基-N-亚硝基胍（MNNG）后，在完全培养基中培养24小时的密度抑制V79细胞与未进行该处理的细胞相比，在所有剂量水平下均显示出更高的存活水平和更低的突变频率。然而，在这24小时培养期间，如果存在聚（ADP-核糖）合成抑制剂苯甲酰胺，则未观察到存活改善和突变频率降低的情况。相反，与对照组相比，细胞对MNNG变得更加敏感，并且在所研究的所有剂量下突变频率也显著增加。
