Hameed Samer Sadeq, Guo Jianhua, Tizard Ian, Shivaprasad H L, Payne Susan
Schubot Exotic Bird Health Center, Texas A&M University, College of Veterinary Medicine and Biomedical Sciences, College Station, TX 77843, USA.
University of California-Davis, CAHFS - Tulare Branch, 18830 Road 112, Tulare, CA 93274, USA.
Virology. 2018 Feb;515:81-91. doi: 10.1016/j.virol.2017.12.007. Epub 2017 Dec 20.
We have demonstrated that vaccination of cockatiels (Nymphicus hollandicus) with killed parrot bornavirus (PaBV) plus recombinant PaBV-4 nucleoprotein (N) in alum was protective against disease in birds challenged with a virulent bornavirus isolate (PaBV-2). Unvaccinated birds, as well as birds vaccinated after challenge, developed gross and histologic lesions typical of proventricular dilatation disease (PDD). There was no evidence that vaccination either before or after challenge made the infection more severe. Birds vaccinated prior to challenge largely remained free of disease, despite the persistence of the virus in many organs. Similar results were obtained when recombinant N, in alum, was used for vaccination. In some rodent models, Borna disease is immune mediated thus we did an additional study whereby cyclosporine A was administered to unvaccinated birds starting 1day prior to challenge. This treatment also conferred complete protection from disease, but not infection.
我们已经证明，用灭活鹦鹉博尔纳病毒（PaBV）加重组PaBV-4核蛋白（N）与明矾混合对鸡尾鹦鹉（虎皮鹦鹉）进行疫苗接种，可保护鸟类免受强毒博尔纳病毒分离株（PaBV-2）攻击后的疾病侵害。未接种疫苗的鸟类，以及在攻击后接种疫苗的鸟类，出现了典型的腺胃扩张病（PDD）肉眼和组织学病变。没有证据表明在攻击前或攻击后接种疫苗会使感染更严重。尽管病毒在许多器官中持续存在，但在攻击前接种疫苗的鸟类在很大程度上仍未患病。当使用重组N与明矾混合进行疫苗接种时，也获得了类似的结果。在一些啮齿动物模型中，博尔纳病是由免疫介导的，因此我们进行了一项额外的研究，从攻击前1天开始，对未接种疫苗的鸟类给予环孢素A。这种治疗也能完全预防疾病，但不能预防感染。
