鸭和鸡脑部感染水生禽正呼肠孤病毒-1（ABBV-1）的转录组分析。

Transcriptome Analysis of Duck and Chicken Brains Infected with Aquatic Bird Bornavirus-1 (ABBV-1).

机构信息

Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

出版信息

Viruses. 2022 Oct 8;14(10):2211. doi: 10.3390/v14102211.

DOI:10.3390/v14102211

PMID:36298766

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9611670/

Abstract

Aquatic bird bornavirus 1 (ABBV-1) is a neurotropic virus that infects waterfowls, resulting in persistent infection. Experimental infection showed that both Muscovy ducks and chickens support persistent ABBV-1 infection in the central nervous system (CNS), up to 12 weeks post-infection (wpi), without the development of clinical disease. The aim of the present study was to describe the transcriptomic profiles in the brains of experimentally infected Muscovy ducks and chickens infected with ABBV-1 at 4 and 12 wpi. Transcribed RNA was sequenced by next-generation sequencing and analyzed by principal component analysis (PCA) and differential gene expression. The functional annotation of differentially expressed genes was evaluated by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The PCA showed that the infected ducks sampled at both 4 and 12 wpi clustered separately from the controls, while only the samples from the chickens at 12 wpi, but not at 4 wpi, formed a separate cluster. In the ducks, more genes were differentially expressed at 4 wpi than 12 wpi, and the majority of the highly differentially expressed genes (DEG) were upregulated. On the other hand, the infected chickens had fewer DEGs at 4 wpi than at 12 wpi, and the majority of those with high numbers of DEGs were downregulated at 4 wpi and upregulated at 12 wpi. The functional annotation showed that the most enriched GO terms were immune-associated in both species; however, the terms associated with the innate immune response were predominantly enriched in the ducks, whereas the chickens had enrichment of both the innate and adaptive immune response. Immune-associated pathways were also enriched according to the KEGG pathway analysis in both species. Overall, the transcriptomic analysis of the duck and chicken brains showed that the main biological responses to ABBV-1 infection were immune-associated and corresponded with the levels of inflammation in the CNS.

摘要

水禽 bornavirus 1（ABBV-1）是一种感染水禽的神经嗜性病毒，导致持续性感染。实验感染表明，番鸭和鸡都能在中枢神经系统（CNS）中支持 ABBV-1 的持续性感染，直至感染后 12 周（wpi），且不发生临床疾病。本研究旨在描述实验感染 ABBV-1 的番鸭和鸡的大脑转录组图谱，分别在感染后 4 和 12 wpi 采样。通过下一代测序对转录 RNA 进行测序，并通过主成分分析（PCA）和差异基因表达进行分析。通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析评估差异表达基因的功能注释。PCA 表明，在 4 和 12 wpi 采样的感染鸭与对照组分开聚类，而只有在 12 wpi 采样的鸡样本形成了单独的聚类，而不是在 4 wpi。在番鸭中，4 wpi 时差异表达的基因多于 12 wpi，且大多数高差异表达基因（DEG）上调。另一方面，感染鸡在 4 wpi 时的 DEG 少于 12 wpi，且在 4 wpi 时下调、在 12 wpi 时上调的高数量 DEG 居多。功能注释表明，在两个物种中，最丰富的 GO 术语与免疫相关；然而，在番鸭中，与先天免疫反应相关的术语占主导地位，而在鸡中，先天和适应性免疫反应都有富集。根据 KEGG 通路分析，免疫相关通路在两个物种中也有富集。总体而言，番鸭和鸡大脑的转录组分析表明，对 ABBV-1 感染的主要生物学反应是与 CNS 炎症水平相关的免疫反应。