Guo Zhenhua, Lv Lei, Liu Di, Fu Bo
Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Animal Husbandry Research Institute, Key Laboratory of Combining Farming and Animal Husbandry, Ministry of Agriculture, No. 368 Xuefu Road, Harbin 150086, PR China; Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture, No. 2 Yuanmingyuanxi Road, Beijing 100193, PR China.
Wood Science Research Institute of Heilongjiang Academy of Forestry, No. 134 Haping Road, Harbin 150080, PR China.
Res Vet Sci. 2018 Apr;117:161-166. doi: 10.1016/j.rvsc.2017.12.011. Epub 2017 Dec 18.
Although somatic cell nuclear transfer (SCNT) can be used to create transgenic pigs for human xenotransplantation, low efficiency limits its use. Trichostatin A (TSA) promotes SCNT embryo development, but whether TSA modifies SCNT blastocyst numbers is unclear. Thus, there is an urgent need to understand whether TSA modifies the rate and number of embryos that grow from oocytes to blastocysts in culture and what types of cell signaling pathways may be involved. Thus, we identified 63 reports, of which 13 are included in this meta-analysis. Data show that TSA significantly increased the SCNT blastocyst formation rate, but did not change blastocyst cell number. Due to study heterogeneity (I>50%), we hypothesized that donor cells were of different backgrounds so we analyzed two donor cell subgroups: fetal and adult fibroblasts. Analysis of the fetal fibroblast subgroups showed no heterogeneity, but the adult fibroblast subgroups were heterogeneous, suggesting epigenetic reprogramming of fetal fibroblasts by TSA. Adult fibroblast heterogeneity may be complex and reprogramming by TSA is more difficult. Thus, TSA fibroblasts reprogramming is the source of heterogeneity in this meta-analysis. More work is needed to better understand how TSA influences SCNT pig embryonic development, and histone deacetylase inhibitors can be assessed with respect to SCNT pig embryos. Finally, efforts in epigenetic research may improve SCNT pig embryo outcomes.
虽然体细胞核移植（SCNT）可用于培育用于人类异种移植的转基因猪，但其低效率限制了它的应用。曲古抑菌素A（TSA）可促进SCNT胚胎发育，但TSA是否会改变SCNT囊胚数量尚不清楚。因此，迫切需要了解TSA是否会改变培养过程中从卵母细胞发育到囊胚的胚胎的速率和数量，以及可能涉及哪些类型的细胞信号通路。因此，我们筛选出63篇报告，其中13篇纳入了本荟萃分析。数据显示，TSA显著提高了SCNT囊胚形成率，但并未改变囊胚细胞数量。由于研究存在异质性（I>50%），我们推测供体细胞背景不同，因此我们分析了两个供体细胞亚组：胎儿和成体成纤维细胞。对胎儿成纤维细胞亚组的分析显示不存在异质性，但成体成纤维细胞亚组存在异质性，这表明TSA对胎儿成纤维细胞进行了表观遗传重编程。成体成纤维细胞的异质性可能较为复杂，TSA对其进行重编程更加困难。因此，TSA对成纤维细胞的重编程是本荟萃分析中异质性的来源。需要开展更多工作以更好地了解TSA如何影响SCNT猪胚胎发育，并且可以针对SCNT猪胚胎评估组蛋白去乙酰化酶抑制剂。最后，表观遗传学研究方面的努力可能会改善SCNT猪胚胎的结果。
