Bardak H, Uğuz A C, Bardak Y
1 Department of Ophthalmology, Haydarpasa Numune Research and Training Hospital , Istanbul, Turkey.
2 Faculty of Medicine, Department of Biophysics, Süleyman Demirel University , Isparta, Turkey.
Physiol Int. 2017 Dec 1;104(4):301-315. doi: 10.1556/2060.104.2017.4.3.
In this study, we aimed to observe whether curcumin (cur), a polyphenolic compound derived from the dietary spice turmeric, a yellow substance obtained from the root of the plant Curcuma longa Linn, has any protective effect against blue light irradiation in human retinal pigment epithelium (ARPE-19) cells. For this purpose, we evaluated the intracellular calcium release mechanism, poly ADP ribose polymerase (PARP), procaspase-3/-9 protein expression levels, caspase activation, and reactive oxygen species levels. ARPE-19 cells were divided into four main groups, such as control, cur, blue light, and cur + blue light. Results were evaluated by Kruskal-Wallis and Mann-Whitney U tests as post hoc tests. The cells in cur and cur + blue light samples were incubated with 20 μM cur. Blue light exposure was performed for 24 h in an incubator. Lipid peroxidation and cytosolic-free Ca [Ca] concentrations were higher in the blue light exposure samples than in the control samples; however, their levels were determined as significantly lower in the cur and cur + blue light exposure samples than in the blue light samples alone. PARP and procaspase-3 levels were significantly higher in blue light samples. Cur administration significantly decreased PARP and procaspase-3 expression levels. Reduced glutathione and glutathione peroxidase values were lower in the blue light exposure samples, although they were higher in the cur and cur + blue light exposure samples. Caspase-3 and -9 activities were lower in the cur samples than in the blue light samples. Moreover, vascular endothelial growth factor (VEGF) levels were significantly higher in the blue light exposure samples. In conclusion, cur strongly induced regulatory effects on oxidative stress, intracellular Ca levels, VEGF levels, PARP expression levels, and caspase-3 and -9 values in an experimental oxidative stress model in ARPE-19 cells.
在本研究中，我们旨在观察姜黄素（Cur），一种从食用香料姜黄中提取的多酚类化合物，姜黄是从植物姜黄（Curcuma longa Linn）的根中获得的黄色物质，是否对人视网膜色素上皮（ARPE - 19）细胞中的蓝光照射具有任何保护作用。为此，我们评估了细胞内钙释放机制、聚ADP核糖聚合酶（PARP）、procaspase - 3/-9蛋白表达水平、半胱天冬酶激活以及活性氧水平。ARPE - 19细胞被分为四个主要组，即对照组、Cur组、蓝光组和Cur +蓝光组。结果通过Kruskal - Wallis和Mann - Whitney U检验作为事后检验进行评估。Cur组和Cur +蓝光组样本中的细胞用20μM的Cur孵育。在培养箱中进行24小时的蓝光照射。蓝光照射样本中的脂质过氧化和胞质游离钙[Ca]浓度高于对照样本；然而，它们的水平在Cur组和Cur +蓝光照射样本中被确定为显著低于仅蓝光样本。蓝光样本中的PARP和procaspase - 3水平显著更高。给予Cur显著降低了PARP和procaspase - 3的表达水平。蓝光照射样本中的还原型谷胱甘肽和谷胱甘肽过氧化物酶值较低，尽管它们在Cur组和Cur +蓝光照射样本中较高。Cur组样本中的半胱天冬酶 - 3和 - 9活性低于蓝光样本。此外，蓝光照射样本中的血管内皮生长因子（VEGF）水平显著更高。总之，在ARPE - 19细胞的实验性氧化应激模型中，Cur对氧化应激、细胞内钙水平、VEGF水平、PARP表达水平以及半胱天冬酶 - 3和 - 9值具有强烈的诱导调节作用。
