Ozses Ozkaya Burcu, Gulsahi Kamran, Ungor Mete, Gocmen Julide Sedef
Oral and Dental Health Center, Hatay, Turkey.
Department of Endodontics, Faculty of Dentistry, Başkent University, Ankara, Turkey.
Scanning. 2017 Nov 27;2017:6215482. doi: 10.1155/2017/6215482. eCollection 2017.
This study evaluated the antimicrobial efficacy of Er:YAG laser activation with photon-initiated photoacoustic streaming (PIPS), Nd:YAG laser disinfection, and conventional irrigation on biofilms using scanning electron microscopy (SEM). Biofilms were grown on 110 root halves and divided into the following: Groups 1 and 2 (saline and 1% NaOCl with apical position of PIPS, resp.), Groups 3 and 4 (saline and 1% NaOCl with coronal position of PIPS, resp.), Groups 5 and 6 (Nd:YAG laser after saline and 1% NaOCl irrigation, resp.) and Groups 7, 8, and 9 (conventional irrigation with 1% NaOCl, 6% NaOCl, and saline, resp.). SEM images of the apical, middle, and coronal levels were examined using a scoring system. Score differences between Groups 1 and 2 were insignificant at all levels in the remaining biofilm. Group 4 had significantly greater bacterial elimination than Group 3 at all levels. Differences in Nd:YAG laser irradiation between Groups 5 and 6 were insignificant. Groups 7 and 8 were insignificantly different, except at the middle level. Saline group had a higher percentage of biofilms than the others. In this study, PIPS activation with NaOCl eliminates more biofilms in all root canals regardless of the position of the fiber tip.
本研究使用扫描电子显微镜（SEM）评估了铒钇铝石榴石（Er:YAG）激光联合光子引发光声流（PIPS）、钕钇铝石榴石（Nd:YAG）激光消毒以及传统冲洗对生物膜的抗菌效果。生物膜在110个半根上生长，并分为以下几组：第1组和第2组（分别为生理盐水和1%次氯酸钠，PIPS位于根尖部位），第3组和第4组（分别为生理盐水和1%次氯酸钠，PIPS位于冠部位置），第5组和第6组（分别在生理盐水和1%次氯酸钠冲洗后进行Nd:YAG激光照射），以及第7组、第8组和第9组（分别用1%次氯酸钠、6%次氯酸钠和生理盐水进行传统冲洗）。使用评分系统检查根尖、中部和冠部水平的SEM图像。在剩余生物膜的所有水平上，第1组和第2组之间的评分差异不显著。在所有水平上，第4组的细菌清除率均显著高于第3组。第5组和第6组之间的Nd:YAG激光照射差异不显著。第7组和第8组除中部水平外差异不显著。生理盐水组的生物膜百分比高于其他组。在本研究中，无论光纤尖端位置如何，NaOCl联合PIPS激活在所有根管中能清除更多生物膜。
