Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York, 11794.
Department of Materials Science and Engineering, Stony Brook University, Stony Brook, New York, 11794.
J Biomed Mater Res A. 2019 Jun;107(6):1143-1153. doi: 10.1002/jbm.a.36606. Epub 2019 Mar 13.
This study investigates the mechanical properties and in vitro cytotoxicity of two-dimensional (2D) graphene oxide nanoribbons and nanoplatelets (GONRs and GONPs) reinforced porous polymeric nanocomposites. Highly porous poly(propylene fumarate) (PPF) nanocomposites were prepared by dispersing 0.2 wt % single- and multiwalled SONRs (SWGONRs and MWGONRs) and GONPs. The mechanical properties of scaffolds were characterized using compression testing and in vitro cytocompatibility was assessed using QuantiFlour assay for cellularity and PrestoBlue assay for cell viability. Immunofluorescence was used to assess collagen-I expression and deposition in the extracellular matrix. Porous PPF scaffolds were used as a baseline control and porous single and multiwalled carbon nanotubes (SWCNTs and MWCNTs) reinforced nanocomposites were used as positive controls. Results show that incorporation of 2D graphene nanomaterials leads to an increase in the mechanical properties of porous PPF nanocomposites with following the trend: MWGONRs > GONPs > SWGONRs > MWCNTs > SWCNTs > PPF control. MWGONRs showed the best enhancement of compressive mechanical properties with increases of up to 26% in compressive modulus (i.e., Young's modulus), ~60% in yield strength, and ~24% in the ultimate compressive strength. Addition of 2D nanomaterials did not alter the cytocompatibility of porous PPF nanocomposites. Furthermore, PPF nanocomposites reinforced with SWGONRs, MWGONRs, and GONPs show an improvement in the adsorption of collagen-I compared to PPF baseline control. The results of this study show that 2D graphene nanomaterial reinforced porous PPF nanocomposites possess superior mechanical properties, cytocompatibility, and increased protein adsorption. The favorable cytocompatibility results opens avenues for in vivo safety and efficacy studies for bone tissue engineering applications. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1143-1153, 2019.
本研究调查了二维（2D）氧化石墨烯纳米带和纳米片（GONRs 和 GONPs）增强多孔聚合物纳米复合材料的力学性能和体外细胞毒性。通过分散 0.2wt%的单壁和多壁 SONRs（SWGONRs 和 MWGONRs）和 GONPs，制备了高度多孔的聚（富马酸丙二醇酯）（PPF）纳米复合材料。通过压缩试验对支架的力学性能进行了表征，并通过 QuantiFlour 法评估细胞活力，通过 PrestoBlue 法评估细胞活力。免疫荧光用于评估细胞外基质中胶原蛋白-I 的表达和沉积。多孔 PPF 支架用作基线对照，多孔单壁和多壁碳纳米管（SWCNTs 和 MWCNTs）增强纳米复合材料用作阳性对照。结果表明，掺入 2D 石墨烯纳米材料可提高多孔 PPF 纳米复合材料的力学性能，其趋势为：MWGONRs>GONPs>SWGONRs>MWCNTs>SWCNTs>PPF 对照。MWGONRs 显示出最佳的抗压力学性能增强，抗压模量（即杨氏模量）提高了 26%，屈服强度提高了约 60%，抗压强度提高了约 24%。添加 2D 纳米材料不会改变多孔 PPF 纳米复合材料的细胞相容性。此外，与 PPF 基线对照相比，SWGONRs、MWGONRs 和 GONPs 增强的 PPF 纳米复合材料对胶原蛋白-I 的吸附能力得到提高。本研究结果表明，2D 石墨烯纳米材料增强的多孔 PPF 纳米复合材料具有优异的力学性能、细胞相容性和增加的蛋白质吸附性。良好的细胞相容性结果为骨组织工程应用的体内安全性和功效研究开辟了道路。© 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1143-1153, 2019.
