Instituto Regional de Investigación Científica Aplicada, Universidad de Castilla-La Mancha (UCLM) , Avenida Camilo Jose Cela s/n, 13071 Ciudad Real, Spain.
Escuela Técnica Superior de Ingenieros (ETSI) Industriales, Universidad de Castilla-La Mancha (UCLM) , Avenida Camilo Jose Cela s/n, 13071 Ciudad Real, Spain.
Anal Chem. 2018 Feb 6;90(3):1542-1546. doi: 10.1021/acs.analchem.7b04114. Epub 2018 Jan 5.
We report the use of a small-volume nuclear-magnetic-resonance (NMR)-spectroscopy device with integrated fiber-optics for the real-time detection of UV-vis-light-assisted chemical reactions. An optical fiber is used to guide the light from LEDs or a laser diode positioned safely outside the magnet toward the 25 nL detection volume and placed right above the microfluidic channel, irradiating the transparent back of the NMR chip. The setup presented here overcomes the limitations of conventional NMR systems for in situ UV-vis illumination, with the microchannel permitting efficient light penetration even in highly concentrated solutions, requiring lower-power light intensities, and enabling high photon flux. The efficacy of the setup is illustrated with two model reactions activated at different wavelengths.
我们报告了一种使用小体积核磁共振（NMR）光谱仪与集成光纤的实时检测紫外可见光辅助化学反应的方法。光纤用于将 LED 或激光二极管发出的光引导至安全置于磁体外的 25nL 检测体积，并放置在微流道正上方，以照射 NMR 芯片的透明背面。这里介绍的设置克服了传统 NMR 系统用于原位紫外可见光照的局限性，微通道即使在高浓度溶液中也能实现高效的光穿透，所需的光强更低，并能实现高光通量。该设置的有效性通过两种在不同波长下激活的模型反应来说明。
