Bramham Jack E, Golovanov Alexander P
Department of Chemistry, School of Natural Sciences, Faculty of Science and Engineering, The University of Manchester, Manchester, M13 9PL, UK.
Commun Chem. 2022 Aug 4;5(1):90. doi: 10.1038/s42004-022-00704-5.
In situ illumination of liquid-state nuclear magnetic resonance (NMR) samples makes it possible for a wide range of light-dependent chemical and biological phenomena to be studied by the powerful analytical technique. However, the position of an NMR sample deep within the bore of the spectrometer magnet renders such illumination challenging. Here, we demonstrate the working principles of a sample illumination device (NMRtorch) where a lighthead containing an LED array is positioned directly at the top of an NMRtorch tube which is inserted into the NMR spectrometer. The wall of the tube itself acts as a light guide, illuminating the sample from the outside. We explore how this new setup performs in a number of photo-NMR applications, including photoisomerisation and photo-chemically induced dynamic nuclear polarisation (photo-CIDNP), and demonstrate the potential for ultraviolet (UV) degradation studies with continuous online NMR assessment. This setup enables users of any typical liquid-state spectrometer to easily perform in situ photo-NMR experiments, using a wide range of wavelengths.
对液态核磁共振（NMR）样品进行原位光照，使得利用这种强大的分析技术研究各种光依赖型化学和生物现象成为可能。然而，NMR样品位于光谱仪磁体 bore 深处的位置使得这种光照具有挑战性。在此，我们展示了一种样品光照装置（NMRtorch）的工作原理，其中一个包含LED阵列的灯头直接位于插入NMR光谱仪的NMRtorch管顶部。管本身的壁充当光导，从外部照亮样品。我们探索了这种新设置在许多光-NMR应用中的表现，包括光异构化和光化学诱导动态核极化（光-CIDNP），并展示了通过连续在线NMR评估进行紫外线（UV）降解研究的潜力。这种设置使任何典型液态光谱仪的用户都能够使用多种波长轻松进行原位光-NMR实验。
