National Key Laboratory of Plant Molecular Genetics, Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
University of Chinese Academy of Sciences, Beijing, China.
PLoS Biol. 2017 Dec 28;15(12):e2002978. doi: 10.1371/journal.pbio.2002978. eCollection 2017 Dec.
Ion homeostasis is essential for plant growth and environmental adaptation, and maintaining ion homeostasis requires the precise regulation of various ion transporters, as well as correct root patterning. However, the mechanisms underlying these processes remain largely elusive. Here, we reported that a choline transporter gene, CTL1, controls ionome homeostasis by regulating the secretory trafficking of proteins required for plasmodesmata (PD) development, as well as the transport of some ion transporters. Map-based cloning studies revealed that CTL1 mutations alter the ion profile of Arabidopsis thaliana. We found that the phenotypes associated with these mutations are caused by a combination of PD defects and ion transporter misregulation. We also established that CTL1 is involved in regulating vesicle trafficking and is thus required for the trafficking of proteins essential for ion transport and PD development. Characterizing choline transporter-like 1 (CTL1) as a new regulator of protein sorting may enable researchers to understand not only ion homeostasis in plants but also vesicle trafficking in general.
离子内稳是植物生长和环境适应的基础，而维持离子内稳需要各种离子转运蛋白的精确调控，以及正确的根系模式形成。然而，这些过程的机制在很大程度上仍不清楚。在这里，我们报道了胆碱转运蛋白基因 CTL1 通过调节质膜通道（PD）发育所需的蛋白质的分泌运输，以及一些离子转运蛋白的运输，来控制离子组内稳。基于图谱的克隆研究表明，CTL1 突变改变了拟南芥的离子谱。我们发现，这些突变相关的表型是由 PD 缺陷和离子转运蛋白调控异常的组合引起的。我们还证实，CTL1 参与调节囊泡运输，因此对于离子运输和 PD 发育所必需的蛋白质的运输是必需的。将胆碱转运蛋白样 1（CTL1）鉴定为一种新的蛋白质分选调节剂，可能使研究人员不仅能够理解植物中的离子内稳，还能够理解一般的囊泡运输。
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