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含华支睾吸虫囊蚴皮蛋白的类病毒颗粒疫苗诱导对感染华支睾吸虫的部分保护作用。

Virus-like particles vaccine containing Clonorchis sinensis tegumental protein induces partial protection against Clonorchis sinensis infection.

机构信息

Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, South Korea.

Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul, South Korea.

出版信息

Parasit Vectors. 2017 Dec 29;10(1):626. doi: 10.1186/s13071-017-2526-5.

DOI:10.1186/s13071-017-2526-5
PMID:29284528
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5747077/
Abstract

BACKGROUND

Human clonorchiasis, caused by the infection of Clonorchis sinensis, is one of the major health problems in Southeast Asia. However, vaccine efficacy against C. sinensis infection remains largely unknown.

METHODS

In this study, for the first time, we generated virus-like particles (VLPs) vaccine containing the C. sinensis tegumental protein 22.3 kDa (CsTP 22.3) and the influenza matrix protein (M1) as a core protein, and investigated the vaccine efficacy in Sprague-Dawley rats.

RESULTS

Intranasal immunization of VLPs vaccine induced C. sinensis-specific IgG, IgG2a and IgG2c in the sera and IgA responses in the feces and intestines. Notably, upon challenge infection with C. sinensis metacercariae, significantly lower adult worm loads (70.2%) were measured in the liver of rats immunized with VLPs, compared to those of naïve rats. Furthermore, VLPs immunization induced antibody secreting cells (ASC) responses and CD4+/CD8+ T cell responses in the spleen.

CONCLUSIONS

Our results indicated that VLPs vaccine containing C. sinensis CsTP 22.3 kDa provided partial protection against C. sisnensis infection. Thus, VLPs could be a potential vaccine candidate against C. sinensis.

摘要

背景

由华支睾吸虫感染引起的人体华支睾吸虫病是东南亚地区的主要健康问题之一。然而，针对华支睾吸虫感染的疫苗效果在很大程度上仍不清楚。

方法

在这项研究中，我们首次制备了含有华支睾吸虫囊蚴表面蛋白 22.3 kDa（CsTP 22.3）和流感基质蛋白（M1）作为核心蛋白的病毒样颗粒（VLPs）疫苗，并在斯普拉格-道利大鼠中评估了疫苗的效果。

结果

鼻内免疫 VLPs 疫苗可诱导血清中产生华支睾吸虫特异性 IgG、IgG2a 和 IgG2c，以及粪便和肠道中的 IgA 反应。值得注意的是，与华支睾吸虫囊蚴感染挑战后相比，VLPs 免疫组大鼠肝脏中的成虫负荷（70.2%）明显降低。此外，VLPs 免疫可诱导脾脏中抗体分泌细胞（ASC）反应和 CD4+/CD8+T 细胞反应。

结论

我们的结果表明，含有华支睾吸虫 CsTP 22.3 kDa 的 VLPs 疫苗可提供针对华支睾吸虫感染的部分保护。因此，VLPs 可能是一种针对华支睾吸虫的潜在疫苗候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/5747077/7dea3eecaf7d/13071_2017_2526_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/5747077/5b37fc2ec4a8/13071_2017_2526_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/5747077/261bc6c9077a/13071_2017_2526_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/5747077/6139596ea597/13071_2017_2526_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/5747077/fd51eb261db9/13071_2017_2526_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/5747077/2b3da4d5f8cc/13071_2017_2526_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/5747077/8ff7992c3515/13071_2017_2526_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/5747077/7dea3eecaf7d/13071_2017_2526_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/5747077/5b37fc2ec4a8/13071_2017_2526_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/5747077/261bc6c9077a/13071_2017_2526_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/5747077/6139596ea597/13071_2017_2526_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/5747077/fd51eb261db9/13071_2017_2526_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/5747077/2b3da4d5f8cc/13071_2017_2526_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/5747077/8ff7992c3515/13071_2017_2526_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cfc/5747077/7dea3eecaf7d/13071_2017_2526_Fig7_HTML.jpg

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