Peng Yanfang, Zhao Yingqian, Ye Taisheng, Zhang Yabing, Wu Zhaoyan, Xia Yukun, Zhang Yingwen
Department of Traditional Chinese Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.
Exp Ther Med. 2017 Dec;14(6):5459-5463. doi: 10.3892/etm.2017.5247. Epub 2017 Oct 2.
Hepatocellular carcinoma (HCC) is one of the most common cancers types. Niu-Huang-Shen (NHS), a Chinese medicine, has been reported to exert antipyretic, anti-inflammatory and vasodilatation effects. However, whether NHS has inhibitory effects on HCC cell phenotypes has remained elusive. In the present study, Cell Counting Kit-8, colony formation, fluorescence-activated cell sorting and Transwell assays were used to evaluate the effect of NHS on cell proliferation, migration and invasion. The results indicated that NHS suppressed cell proliferation and invasion, inhibited cell apoptosis, and induced cell cycle arrest. In addition, NHS significantly suppressed the mRNA and protein expression of Yes-associated protein (YAP). It was concluded that NHS downregulated YAP expression and inhibited the Hippo signaling pathway as well as HCC cell growth and invasion. NHS may be a novel potential therapeutic for HCC patients.
肝细胞癌（HCC）是最常见的癌症类型之一。中药牛黄参（NHS）据报道具有解热、抗炎和血管舒张作用。然而，NHS对肝癌细胞表型是否具有抑制作用仍不清楚。在本研究中，使用细胞计数试剂盒-8、集落形成、荧光激活细胞分选和Transwell实验来评估NHS对细胞增殖、迁移和侵袭的影响。结果表明，NHS抑制细胞增殖和侵袭，抑制细胞凋亡，并诱导细胞周期停滞。此外，NHS显著抑制Yes相关蛋白（YAP）的mRNA和蛋白表达。得出的结论是，NHS下调YAP表达并抑制Hippo信号通路以及肝癌细胞的生长和侵袭。NHS可能是肝癌患者一种新的潜在治疗方法。
