Department of Urology, The Affiliated Taizhou People's Hospital of Nantong University, Taizhou, Jiangsu 225300, P.R. China.
Mol Med Rep. 2018 Mar;17(3):3783-3788. doi: 10.3892/mmr.2017.8352. Epub 2017 Dec 27.
The role of yes-associated protein (YAP) in human prostate cancer DU145 cells and its underlying molecular mechanisms were explored in the present study. Initially, the expression levels of YAP were detected in DU145 cells, which revealed that YAP was highly expressed in these cells. To investigate the role of YAP in DU145 cells, a stable YAP‑silenced DU145 cell line was generated using YAP‑small interfering RNA. Reverse transcription‑quantitative polymerase chain reaction and western blotting were performed for mRNA and protein detection, respectively. An MTT assay and flow cytometry were performed to investigate the proliferation and apoptosis of DU145 cells. The results demonstrated that YAP knockdown significantly decreased the proliferative ability of DU145 cells, whereas the percentage of apoptotic cells was markedly increased, compared with the control. In addition, the mRNA and protein expression levels of connective tissue growth factor and cysteine‑rich angiogenic factor 61 were notably decreased, the ratio of B‑cell lymphoma 2 (Bcl‑2)/Bcl‑2‑associated X protein (Bax) was significantly reduced, and the expression levels of caspase 3 were significantly decreased within YAP‑silenced DU145 cells. In conclusion, YAP knockdown reduced the proliferation and induced apoptosis of DU145 cells. Therefore, the gene transcription and protein expression of YAP may be involved in the development of prostate cancer and may be considered a potential target for the treatment of such cancers.
本研究探讨了 yes 相关蛋白（YAP）在人前列腺癌 DU145 细胞中的作用及其潜在的分子机制。首先，检测了 DU145 细胞中 YAP 的表达水平，结果显示 YAP 在这些细胞中高表达。为了研究 YAP 在 DU145 细胞中的作用，使用 YAP 小干扰 RNA 构建了稳定沉默 YAP 的 DU145 细胞系。分别通过逆转录-定量聚合酶链反应和蛋白质印迹法进行 mRNA 和蛋白质检测。通过 MTT assay 和流式细胞术分别研究 DU145 细胞的增殖和凋亡。结果表明，与对照组相比，YAP 敲低显著降低了 DU145 细胞的增殖能力，而凋亡细胞的百分比明显增加。此外，结缔组织生长因子和富含半胱氨酸的血管生成因子 61 的 mRNA 和蛋白表达水平明显降低，B 细胞淋巴瘤 2（Bcl-2）/Bcl-2 相关 X 蛋白（Bax）的比值显著降低，YAP 沉默的 DU145 细胞中 caspase 3 的表达水平也明显降低。综上所述，YAP 敲低可降低 DU145 细胞的增殖并诱导其凋亡。因此，YAP 的基因转录和蛋白表达可能参与了前列腺癌的发生发展，可被视为治疗此类癌症的潜在靶点。
