Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, P.R. China.
Oncol Rep. 2018 Mar;39(3):1191-1196. doi: 10.3892/or.2017.6158. Epub 2017 Dec 15.
Chemotherapy resistance of advanced ovarian cancers is responsible for death of most cancer patients, so it is necessary to seek safe and effective natural ingredients to lower the chemotherapy resistance of ovarian cancer. In the present study, we studied the anticancer effects of triptolide (TPL) and TPL + cisplatin (DDP) in vitro and in vivo using SKOV3/DDP cell line and a mouse model. In vitro results showed that TPL and TPL + DDP inhibited cellular invasion and migration of SKOV3/DDP cells (P<0.05), and significantly reduced the expression of adhesion-related proteins integrin β1 (ITGβ1) and apoptosis-inhibiting proteins survivin, matrix metalloproteinase 2 (MMP-2) and MMP-9 (P<0.05). Animal results demonstrated that TPL and TPL + DDP had significantly enhanced the inflammatory factor-2 (IL-2) and tumor necrosis factor-α (TNF-α) in serum of mice, and significantly increased the NK cell-related protein levels of CD16 and CD56, while significantly inhibited the production of vascular endothelial growth factor (VEGF) related protein clusters of differentiation 31 (CD31) and CD105. Collectively, the combination of TPL and DDP may produce a synergistic anticancer effect on epithelial ovarian cancer (EOC).
晚期卵巢癌的化疗耐药性是导致大多数癌症患者死亡的原因，因此有必要寻找安全有效的天然成分来降低卵巢癌的化疗耐药性。在本研究中，我们使用 SKOV3/DDP 细胞系和小鼠模型研究了雷公藤红素（TPL）和 TPL+顺铂（DDP）的体内外抗癌作用。体外结果表明，TPL 和 TPL+DDP 抑制了 SKOV3/DDP 细胞的细胞侵袭和迁移（P<0.05），并显著降低了黏附相关蛋白整合素β1（ITGβ1）和凋亡抑制蛋白生存素、基质金属蛋白酶 2（MMP-2）和 MMP-9 的表达（P<0.05）。动物实验结果表明，TPL 和 TPL+DDP 显著增强了小鼠血清中的炎症因子 2（IL-2）和肿瘤坏死因子-α（TNF-α），显著增加了 NK 细胞相关蛋白 CD16 和 CD56 的水平，同时显著抑制了血管内皮生长因子（VEGF）相关蛋白簇分化 31（CD31）和 CD105 的产生。综上所述，TPL 和 DDP 的联合应用可能对上皮性卵巢癌（EOC）产生协同抗癌作用。
