Fan Yanyan, Zhao Qiqi, Duan Huimin, Bi Shuxin, Hao Xiaomin, Xu Rui, Bai Runyao, Yu Ruonan, Lu Wenting, Bao Tiejun, Wuriyanghan Hada
Key Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China.
Key Laboratory of Ecology and Resource Use of the Mongolian Plateau, Ministry of Education, School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China.
iScience. 2022 Dec 27;26(1):105880. doi: 10.1016/j.isci.2022.105880. eCollection 2023 Jan 20.
Exchanges of mRNA were shown between host and stem parasites but not root parasites. (Orobanchaceae) is a holoparasitic herb which parasitizes on the roots of woody plant (Chenopodiaceae). We used transcriptome sequencing and bioinformatic analyses to identify nearly ten thousand mobile mRNAs. Transcript abundance appears to be a driving force for transfer event and mRNA exchanges occur through haustorial junction. Mobility of selected mRNAs was confirmed and in sunflower- heterologous parasitic system. Four . →. mobile mRNAs appear to facilitate haustorium development. Of interest, two mobile mRNAs of putative resistance genes and cause root-specific hypersensitive response and retard parasite development, which might contribute to parasitic equilibrium. The present study provides evidence for the large-scale mRNA transfer event between a woody host and a root parasite, and demonstrates the functional relevance of six . genes in host-parasite interactions.
研究表明，宿主与茎寄生植物之间存在mRNA交换，但根寄生植物之间不存在这种交换。列当属（列当科）是一种全寄生草本植物，寄生于木本植物（藜科）的根部。我们使用转录组测序和生物信息学分析来鉴定近一万种可移动的mRNA。转录本丰度似乎是转移事件的驱动力，mRNA交换通过吸器连接发生。在向日葵-异源寄生系统中证实了所选mRNA的移动性。四个可移动的mRNA似乎促进了吸器的发育。有趣的是，两个假定抗性基因的可移动mRNA会引起根部特异性超敏反应并延缓寄生虫的发育，这可能有助于寄生平衡。本研究为木本宿主与根寄生植物之间大规模的mRNA转移事件提供了证据，并证明了六个基因在宿主-寄生虫相互作用中的功能相关性。
