Seo Dongmin, Oh Sangwoo, Lee Moonjin, Hwang Yongha, Seo Sungkyu
Department of Electronics and Information Engineering, Korea University, Sejong 30019, Korea.
Maritime Safety Research Division, Korea Research Institute of Ships & Ocean Engineering, Daejeon 34103, Korea.
Sensors (Basel). 2017 Dec 29;18(1):85. doi: 10.3390/s18010085.
This paper demonstrates a commercial-level field-portable lens-free cell analyzer called the NaviCell (No-stain and Automated Versatile Innovative cell analyzer) capable of automatically analyzing cell count and viability without employing an optical microscope and reagents. Based on the lens-free shadow imaging technique, the NaviCell (162 × 135 × 138 mm³ and 1.02 kg) has the advantage of providing analysis results with improved standard deviation between measurement results, owing to its large field of view. Importantly, the cell counting and viability testing can be analyzed without the use of any reagent, thereby simplifying the measurement procedure and reducing potential errors during sample preparation. In this study, the performance of the NaviCell for cell counting and viability testing was demonstrated using 13 and six cell lines, respectively. Based on the results of the hemocytometer ( standard), the error rate (ER) and coefficient of variation (CV) of the NaviCell are approximately 3.27 and 2.16 times better than the commercial cell counter, respectively. The cell viability testing of the NaviCell also showed an ER and CV performance improvement of 5.09 and 1.8 times, respectively, demonstrating sufficient potential in the field of cell analysis.
本文展示了一种名为NaviCell（无染色自动通用创新型细胞分析仪）的商业级现场便携式无透镜细胞分析仪，它无需使用光学显微镜和试剂就能自动分析细胞数量和活力。基于无透镜阴影成像技术，NaviCell（尺寸为162×135×138立方毫米，重量为1.02千克）具有因视野大而使测量结果之间的标准偏差得到改善从而提供分析结果的优势。重要的是，无需使用任何试剂就能分析细胞计数和活力测试，从而简化了测量程序并减少了样品制备过程中的潜在误差。在本研究中，分别使用13种和6种细胞系展示了NaviCell在细胞计数和活力测试方面的性能。基于血细胞计数器（标准）的结果，NaviCell的错误率（ER）和变异系数（CV）分别比商用细胞计数器好约3.27倍和2.16倍。NaviCell的细胞活力测试还显示ER和CV性能分别提高了5.09倍和1.8倍，表明在细胞分析领域具有足够的潜力。
