TIP60瞬时缺失后乳腺上皮细胞迁移的实时成像
Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60.
作者信息
Zhang Yanzhou, Chia Grace SuShin, Tham Cheng Yong, Jha Sudhakar
机构信息
Cancer Science Institute of Singapore, National University of Singapore.
Cancer Science Institute of Singapore, National University of Singapore; Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore;
出版信息
J Vis Exp. 2017 Dec 7(130):56248. doi: 10.3791/56248.
The wound-healing assay is efficient and one of the most economical ways to study cell migration in vitro. Conventionally, images are taken at the beginning and end of an experiment using a phase-contrast microscope, and the migration abilities of cells are evaluated by the closure of wounds. However, cell movement is a dynamic phenomenon, and a conventional method does not allow for tracking single-cell movement. To improve current wound-healing assays, we use live-cell imaging techniques to monitor cell migration in real time. This method allows us to determine the cell migration rate based on a cell tracking system and provides a clearer distinction between cell migration and cell proliferation. Here, we demonstrate the use of live-cell imaging in wound-healing assays to study the different migration abilities of breast epithelial cells influenced by the presence of TIP60. As cell motility is highly dynamic, our method provides more insights into the processes of wound healing than a snapshot of wound closure taken with the traditional imaging techniques used for wound-healing assays.
伤口愈合实验是一种高效且最经济的体外研究细胞迁移的方法之一。传统上，在实验开始和结束时使用相差显微镜拍摄图像，并通过伤口闭合来评估细胞的迁移能力。然而，细胞运动是一个动态现象，传统方法无法追踪单细胞的运动。为了改进当前的伤口愈合实验，我们使用活细胞成像技术实时监测细胞迁移。这种方法使我们能够基于细胞追踪系统确定细胞迁移速率，并更清晰地区分细胞迁移和细胞增殖。在此，我们展示了在伤口愈合实验中使用活细胞成像来研究受TIP60存在影响的乳腺上皮细胞的不同迁移能力。由于细胞运动性是高度动态的，我们的方法比用于伤口愈合实验的传统成像技术拍摄的伤口闭合快照能提供更多关于伤口愈合过程的见解。
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