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本文引用的文献

1
Carbon ion irradiation withstands cancer stem cells' migration/invasion process in Head and Neck Squamous Cell Carcinoma (HNSCC).碳离子辐射可抑制头颈部鳞状细胞癌（HNSCC）中癌症干细胞的迁移/侵袭过程。
Oncotarget. 2016 Jul 26;7(30):47738-47749. doi: 10.18632/oncotarget.10281.
2
Isolation and Characterization of a Head and Neck Squamous Cell Carcinoma Subpopulation Having Stem Cell Characteristics.具有干细胞特征的头颈部鳞状细胞癌亚群的分离与鉴定
J Vis Exp. 2016 May 11(111):53958. doi: 10.3791/53958.
3
[Technical recommendations and best practice guidelines for May-Grünwald-Giemsa staining: literature review and insights from the quality assurance].[May-Grünwald-Giemsa染色的技术建议和最佳实践指南：文献综述与质量保证见解]
Ann Pathol. 2015 Aug;35(4):294-305. doi: 10.1016/j.annpat.2015.05.019. Epub 2015 Jul 16.
4
Radiation-enhanced cell migration/invasion process: a review.辐射增强的细胞迁移/侵袭过程：综述
Crit Rev Oncol Hematol. 2014 Nov;92(2):133-42. doi: 10.1016/j.critrevonc.2014.05.006. Epub 2014 May 22.
5
Epithelial to mesenchymal transition in head and neck squamous cell carcinoma.头颈部鳞状细胞癌中的上皮间质转化。
Oral Oncol. 2013 Apr;49(4):287-92. doi: 10.1016/j.oraloncology.2012.10.009. Epub 2012 Nov 19.
6
Epithelial-to-mesenchymal transition and cancer stem(-like) cells in head and neck squamous cell carcinoma.头颈部鳞状细胞癌中的上皮-间充质转化和癌症干细胞（样）。
Cancer Lett. 2013 Sep 10;338(1):47-56. doi: 10.1016/j.canlet.2012.06.013. Epub 2012 Jul 4.
7
Targeting a cornerstone of radiation resistance: cancer stem cell.靶向辐射抵抗的基石：癌症干细胞。
Cancer Lett. 2012 Sep 28;322(2):139-47. doi: 10.1016/j.canlet.2012.03.024. Epub 2012 Mar 27.
8
Wound-healing assay.伤口愈合试验。
Methods Mol Biol. 2005;294:23-9. doi: 10.1385/1-59259-860-9:023.
9
Boyden chamber assay.博伊登小室试验
Methods Mol Biol. 2005;294:15-22. doi: 10.1385/1-59259-860-9:015.
10
Tumour-cell invasion and migration: diversity and escape mechanisms.肿瘤细胞侵袭与迁移：多样性及逃逸机制
Nat Rev Cancer. 2003 May;3(5):362-74. doi: 10.1038/nrc1075.

细胞侵袭和迁移过程的评估：基于视频显微镜的划痕试验与博伊登室试验的比较

Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay.

作者信息

Guy Jean-Baptiste, Espenel Sophie, Vallard Alexis, Battiston-Montagne Priscillia, Wozny Anne-Sophie, Ardail Dominique, Alphonse Gersende, Rancoule Chloé, Rodriguez-Lafrasse Claire, Magne Nicolas

机构信息

UMR CNRS 5822 /IN2P3, IPNL, PRISME, Laboratoire de Radiobiologie Cellulaire et Moléculaire, Faculté de Médecine Lyon-Sud, Université Lyon 1; Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth.

UMR CNRS 5822 /IN2P3, IPNL, PRISME, Laboratoire de Radiobiologie Cellulaire et Moléculaire, Faculté de Médecine Lyon-Sud, Université Lyon 1.

出版信息

J Vis Exp. 2017 Nov 17(129):56337. doi: 10.3791/56337.

DOI:10.3791/56337
PMID:29286429
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5755419/
Abstract

The invasion and migration abilities of tumor cells are main contributors to cancer progression and recurrence. Many studies have explored the migration and invasion abilities to understand how cancer cells disseminate, with the aim of developing new treatment strategies. Analysis of the cellular and molecular basis of these abilities has led to the characterization of cell mobility and the physicochemical properties of the cytoskeleton and cellular microenvironment. For many years, the Boyden chamber assay and the scratch wound assay have been the standard techniques to study cell invasion and migration. However, these two techniques have limitations. The Boyden chamber assay is difficult and time consuming, and the scratch wound assay has low reproducibility. Development of modern technologies, especially in microscopy, has increased the reproducibility of the scratch wound assay. Using powerful analysis systems, an "in-incubator" video microscope can be used to provide automatic and real-time analysis of cell migration and invasion. The aim of this paper is to report and compare the two assays used to study cell invasion and migration: the Boyden chamber assay and an optimized in vitro video microscope-based scratch wound assay.

摘要

肿瘤细胞的侵袭和迁移能力是癌症进展和复发的主要因素。许多研究探讨了迁移和侵袭能力，以了解癌细胞如何扩散，目的是开发新的治疗策略。对这些能力的细胞和分子基础的分析导致了细胞迁移性以及细胞骨架和细胞微环境的物理化学性质的表征。多年来，Boyden小室试验和划痕试验一直是研究细胞侵袭和迁移的标准技术。然而，这两种技术都有局限性。Boyden小室试验困难且耗时，划痕试验的可重复性较低。现代技术的发展，尤其是显微镜技术的发展，提高了划痕试验的可重复性。使用强大的分析系统，“培养箱内”视频显微镜可用于对细胞迁移和侵袭进行自动实时分析。本文的目的是报告和比较用于研究细胞侵袭和迁移的两种试验：Boyden小室试验和基于体外视频显微镜的优化划痕试验。